Figure 7 : TRiC/CCT is required for the longevity phenotype of long-lived C. elegans mutants.

From: Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan

Figure 7

(a) Knockdown of cct-8 decreases lifespan of IIS (daf-2) and DR (eat-2) long-lived mutant worms at 20 °C (log rank, P<0.0001). N2 (wild-type worms)+vector RNAi: median=17, n=72/9; N2+cct-8 RNAi: median=19, n=66/96; eat-2(ad1116)+vector RNAi: median=23, n=66/96; eat-2(ad1116)+cct-8 RNAi: median=18, n=67/96; daf-2(e1370)+vector RNAi: median=37, n=68/96; daf-2(e1370)+cct-8 RNAi: median=31, n=77/96. (b) Knockdown of cct-8 decreases lifespan of germline-lacking mutant worms (log rank, P<0.0001). glp-1(e2141)+vector RNAi: median=21, n=88/96; glp-1(e2141)+cct-8 RNAi: median=16, n=86/96. (c) Gonad and intestine immunostaining with CCT-1 antibody of wild-type and germline-lacking (glp-1(e2141)) worms. Long-lived germline-lacking nematodes have increased levels of CCT-1 in the intestine compared with wild-type worms. Cell nuclei were stained with DAPI. White arrow indicates gonad and red arrow indicates intestine. DIC, differential interference contrast. Scale bar represents 50 μm. (d) Representative images of GFP expressed under control of the cct-8 promoter in adult wild-type and germline-lacking (glp-1(e2141)) worms. Scale bar represents 100 μm. In c,d the images are representative of three independent experiments. See Supplementary Data 3 for statistical analysis and replicate data of lifespan experiments.