Figure 2 : Ectopic expression of CCT8 is sufficient to increase TRiC/CCT assembly.

From: Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan

Figure 2

(a) Native gel electrophoresis of H9 hESCs and NPCs extracts followed by immunoblotting with CCT antibodies. Extracts were resolved by SDS–PAGE and immunoblotting for analysis of total CCT subunit levels and β-actin loading control. The images are representative of three independent experiments. (b) Label-free quantification (LFQ) of CCT protein levels relative to CCT1. All detected CCT subunits were quantified by their log2 fold change in LFQ intensities relative to CCT1. Graphs represent the mean±s.e.m. (hESCs (n=9), NPCs (n=6)). All the statistical comparisons were made by Student's t-test for unpaired samples. P-value: *P<0.05, **P<0.01, ***P<0.001. (c) Native gel electrophoresis of HEK293T cell extracts followed by immunoblotting with CCT1 antibody (two different exposure times of the same membrane are shown). Extracts were resolved by SDS–PAGE and immunoblotting for analysis of total CCT8 and CCT1 subunit levels. β-actin is the loading control. The images are representative of three independent experiments.