Figure 1 : The expression of CCT subunits decreases during differentiation.

From: Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan

Figure 1

(a,c,e) Western blot analysis with antibodies to CCT8, CCT7, CCT6A, CCT2 and CCT1. β-actin is the loading control. The images are representative of two independent experiments. In a,c the differentiation was performed with the H9 hESC line whereas in e we used H1 hESCs. (b) CCT subunits relative expression to H9 hESCs represents the mean±s.e.m. of three independent experiments. (d) CCT subunits relative expression to H9 hESCs represents the mean±s.e.m. (n=7 independent experiments). (f) CCT subunits relative expression to H1 hESCs represents the mean±s.e.m. of three independent experiments. (g) The protein levels of CCT subunits do not differ with passage in H9 hESCs. β-actin is the loading control. The images are representative of two independent experiments. (h) Data represent the mean±s.e.m. of the relative expression levels to H9 hESCs passage 31–33 (n=3 independent experiments). All the statistical comparisons were made by Student’s t test for unpaired samples. P value: *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.