The relative expression of (a) auxin transport-related genes, (b) auxin synthesis-related genes and (c) auxin signalling-related genes in seeds of Col, snl1, snl2 and snl1snl2 measured by qRT–PCR. Each experiment had three biological repeats and the average value is shown with s.e. Transcript levels were normalized to the level of ACTIN8. The relative transcript level in Col is set as one. The seeds for RNA isolation were fully after-ripened and imbibed for 0, 8 and 24 h.