Figure 5 : Treating mice according to the barcode analysis.

From: Theranostic barcoded nanoparticles for personalized cancer medicine

Figure 5

Based on the barcoded nanoparticle drug screen, a treatment protocol was devised. Mice bearing triple-negative breast cancer tumours were administered doxorubicin, gemcitabine, cisplatin or saline (control). Tumour growth (a) was recorded and postmortem resection (c) and histology (b) of each of the groups was performed. Each group received a therapeutic weekly dose of chemotherapy—doxorubicin (5 mg kg−1), cisplatin (6 mg kg−1) or gemcitabine (125 mg kg−1), while the control group was administered saline. The tumours were resected 23 days after starting the treatment. Tissue slides (b) were immunohistochemically stained with rabbit monoclonal anti-Ki67 antibody to compare the proliferation rate of each group. These show a reduction in the proliferation rate in the gemcitabine-treated tumours compared with the other groups. The data were calculated as the mean±s.e.m. of n=6 per group; *P<0.01; ****P<0.0001. Differences between the two means were tested using an unpaired, two-sided Student's t-test. The efficacy of the treatment is also shown in the tumour size (d), thereby indicating the potency of the treatment. Before introducing the treatment, all the mice had the same average tumour size.