Figure 4 : Expression of IL-4 and IFNγ and IL-17 in iNKT cells or their subsets.

From: Distinct gene expression patterns correlate with developmental and functional traits of iNKT subsets

Figure 4

(a) mRNA expression profiles resulting from transcriptome analyses as depicted in Fig. 1e,f (mean±s.d., n=2). ND, none detected. (b) Total fractions of iNKT cells sorted from thymi of BALB/c or B6 mice, gated as shown in Fig. 1b. Sorted cells were kept either on ice (control) or stimulated with PMA/ionomycin in vitro in the presence of Brefeldin A (stimulated) before intracellular detection of the indicated cytokines by flow cytometry. (c) Detection of cytokines as described in b but iNKT cells from each individual thymus were sorted into iNKT1, 2, and 17 cells, respectively, by gating as shown in Fig. 1b. Pixel sizes in the plots to the right were increased to improve visibility. Data are representative of at least 4 (b) and 2 (c) independent experiments analyzing at least 4 (b) and 3 (c) individual thymi.