Figure 6: Oleic acid attenuates painful and pruritic behaviours. | Nature Communications

Figure 6: Oleic acid attenuates painful and pruritic behaviours.

From: Inhibition of TRPV1 channels by a naturally occurring omega-9 fatty acid reduces pain and itch

Figure 6

(a) Representative TRPV1 currents (as obtained as in Fig. 2) from an inside-out patch from DRG neuron before (grey traces) and after exposure to 4 μM capsaicin (black traces). After wash, 5 μM OA was added for 5 min and the patch was re-exposed to capsaicin (magenta traces). (b) The fraction of uninhibited currents was 13±3.6%, as obtained by normalizing the data obtained in the presence of capsaicin after OA to the data obtained with capsaicin before OA (n=9). (c) Average paw-licking times were: 16.7±2.9 s for capsaicin and 12.1±1.9 s for LPA vehicle injections (Ctrl; n=3–7); 15.0±1.8 s for OA (n=10); 39.4±2.1 for capsaicin (n=5) and 43±1.6 s for LPA (n=9); 24.4±2 s for Caps+OA (n=5) and 27.2±2.9 s for LPA+OA (n=8). *P<0.001 and **P<0.0001 between groups as compared by the brackets. Also see Supplementary Table 2. (d) The average number of scratching bouts were: 11.6±1.1 for vehicle-injected (Ctrl; n=10); 7.3±1.6 for OA (n=10); 31.6±2.7 for cPA (n=16); 17.3±3.2 for cPA+OA (n=7) for WT animals and 6.7±1.7 for control (n=10) and 11.1±2.1 for cPA-injected (n=9) Trpv1−/− mice. For WT mice: *P<0.0001 and **P<0.01 between groups as compared by the brackets. Also see Supplementary Table 3.

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