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# Multi-omic data integration enables discovery of hidden biological regularities

## Abstract

Rapid growth in size and complexity of biological data sets has led to the ‘Big Data to Knowledge’ challenge. We develop advanced data integration methods for multi-level analysis of genomic, transcriptomic, ribosomal profiling, proteomic and fluxomic data. First, we show that pairwise integration of primary omics data reveals regularities that tie cellular processes together in Escherichia coli: the number of protein molecules made per mRNA transcript and the number of ribosomes required per translated protein molecule. Second, we show that genome-scale models, based on genomic and bibliomic data, enable quantitative synchronization of disparate data types. Integrating omics data with models enabled the discovery of two novel regularities: condition invariant in vivo turnover rates of enzymes and the correlation of protein structural motifs and translational pausing. These regularities can be formally represented in a computable format allowing for coherent interpretation and prediction of fitness and selection that underlies cellular physiology.

## Introduction

Progress of the biological sciences in the era of big data will depend on how we address the following question: ‘How do we connect multiple disparate data types1 to obtain a meaningful understanding of the biological functions of an organism2?’ Owing to large-scale improvements in omics technologies, we can now quantitatively track changes in biological processes in unprecedented detail3,4. Although such measurements span a diverse range of cellular activities, developing an understanding of how these data types quantitatively relate to one another and to the phenotypic characteristics of the organism remains elusive. This issue is central to the so-called Big Data to Knowledge (BD2K) grand challenge, which aims to integrate multiple disparate data types into a biologically meaningful, multi-level structure1,2.

Interpretation of disparate data requires understanding how the primary measurements of different omics data are quantitatively coupled to one another5. We approach this task by identifying regularities (relationships between biological data types that remain relatively constant across conditions) between pairwise omics data types. Although some regularities can readily be discovered through direct pairwise omics data comparisons, we find that other regularities emerge only through more intricate analysis leveraged by mechanistically based network reconstructions6. Such reconstructions can be used as a context for poly-omic data integration and analysis6,7, and, when combined with constraint-based modelling approaches8,9, provide important links between omics data and phenotypic characteristics of the organism.

As we will show, this approach leads to a comprehensive synchronization of poly-omic data with computed growth states. The approach directly addresses the BD2K grand challenge and is made conceptually accessible by tracing the ‘information flow’ through the familiar ‘central dogma’, to establish relationships between measurements and cell physiology (Fig. 1).

## Results

### Pairwise ratios of data types are highly correlated

First, we examine the information flow from transcription to translation, to protein production, by identifying correlations across primary omics data types, such as RNAseq10, ribosome profiling11,12,13 and proteomics14, collected for Escherichia coli batch growth on glucose, fumarate, pyruvate and acetate (Fig. 1, ‘primary data box’). We found relatively poor correlations of messenger RNA to protein across conditions (r2<0.4), consistent with previous studies15,16. Stronger correlations (r2>0.8) emerge when analysing the ratio of protein per mRNA (ρPM) on a per-gene basis (the difference between peptide abundance and relative mRNA read counts per gene for multiple growth conditions; Supplementary Fig. 1a). Computing the median coefficient of variation shows that changes in ρPM across conditions are relatively invariant. In addition, we find the number of ribosomes required (ribosome occupancy of mRNA) per protein translated is also relatively invariant across all four conditions (r2>0.7; Supplementary Fig. 1b).

### Translation rate is linked to protein secondary structure

Second, we examined pairwise relationships between other omics data types, such as ribosome profiling, proteomics and fluxomics, by integrating these data types into next-generation genome-scale models (Fig. 1, ‘integration with genome-scale models of metabolism (GEMs) box’). GEMs are based on the annotated sequence and analysis of the bibliome for functionally annotated gene products6. The most recent generations of genome-scale models incorporate protein structural information17,18 and allow for the computation of the synthesis of the entire proteome of a cell in addition to the balanced use of its metabolic network19. These models can integrate multiple layers of biological organization to balance the use of all cellular components, to achieve a cellular state. It can thus extend our understanding of how information flows from translation to protein folding and catalysis, and its role in producing whole-cell functions.

We examined how information flows during protein translation, which includes protein folding. Recent studies indicate a possible link between translation speed and proper folding12,20. Analysis of translational pausing has typically been approached from a sequence-based viewpoint20. Here we approach this analysis from a different perspective, by correlating the occurrence of translational pausing on a transcript to the location of nearby protein secondary (2°) structure motifs (Fig. 2). The establishment of this correlation is based on the following: (1) ribosome profiling11,12,13, which provides ample information on the queuing of ribosomes along mRNA transcripts; and (2) a recent network reconstruction that contains comprehensive protein structural information linked to the translated protein at the proteome scale18.

Several striking regularities in translational pausing and protein structure are consistently observed across multiple growth conditions in E. coli, which suggest the co-translational folding of intermediate secondary structure motifs inside the ribosome exit tunnel (Fig. 2a). We find that pause sites are enriched (p-value<0.01 using a hypergeometric test) downstream of specific secondary structure motifs, such as α-helices and β-sheets (Fig. 2b and Supplementary Fig. 2), yet are not significantly enriched at the termini of domains (Supplementary Fig. 3 and also see Supplementary Note 1). On average, pausing becomes most substantial six to eight amino acids downstream of α-helices and β-sheets, which, in the majority of cases, fall either on disordered regions of the protein or on helical residues. Such instances consistently account for >35–40% of pause sites across different conditions (Fig. 2c and Supplementary Fig. 4). These findings strongly corroborate a growing theory that partially folded intermediate protein structures begin to immediately fold inside the ribosome exit tunnel, following polypeptide-chain synthesis. Several previous studies have shown that partially folded protein structures, such as small domains, can be detected within the exit tunnel21,22,23. More recently, Nilsson et al.24 demonstrated the co-translational folding of a small zinc finger-like domain deep within the ribosome exit tunnel using arrest-peptide-mediated force measurements in conjunction with cryo-electron tomography.

Do sequence-specific motifs drive co-translational pausing to ensure proper protein folding? We find that Shine–Dalgarno (SD)-like sequences account for 20–22% of ribosome density at pause sites (Fig. 2c and see ‘Identification of SD-like codons’ in Methods), which is consistent with recent studies25, and four times less frequent than what is found previous studies20. Of the pausing instances linked to SD-like sequences, we find that, on average, nearly half of these pausing regions also fall in the nearby vicinity (five to ten codons) of helices or sheets. The link between pausing, SD-like sequence and protein secondary structure becomes clear when comparing the average occurrence of SD-like sequences genome-wide (9%) with their occurrence directly downstream of α-helices (35%) and β-sheets (18%, Fig. 2d). Together, these sequence and structure motifs account for the majority of pause sites (60%) or nearly half of the total ribosome occupancy (Supplementary Fig. 4). These findings suggest that co-translational pausing occurs for distinct secondary structural elements and supports the potential role of sequence-specific factors to drive pausing for ensuring proper protein folding (Fig. 2e).

### Genome-wide estimation of enzyme turnover rates

How does information flow between an individual enzyme’s catalytic activity and the activity of an entire network? To evaluate the effective turnover rate of enzymes, reaction flux per enzyme can be directly computed using experimental values for both flux (the rate of reactions) and enzyme abundance26 on a small scale (mainly for central carbon metabolism). To assess enzyme turnover on a genome scale, we computed the ratio of an enzyme’s abundance (measured from proteomics data) and its corresponding flux derived from network-based analyses using the iOL1650-ME model (Supplementary Note 2 and Fig. 3). As the iOL1650-ME model directly relates enzyme synthesis and metabolic flux, we were able to develop a method, which uses the model to extrapolate the most probable flux state from proteomics. These ratios quantitatively couple experimentally derived flux estimates and protein abundances to make a quantitative connection between data types.

Estimates of enzyme turnover rates (keff), which represent coupling coefficients between the fluxome and the proteome (Supplementary Note 3), were analysed across four nutrient conditions, to understand the effect that carbon uptake has on metabolic enzyme turnover rates. We find that these parameters show considerable regularity in relating flux to protein abundance, which suggests that in vivo turnover rate for most enzymes does not strongly depend on growth in diverse batch culture settings (Supplementary Note 4). For high-flux metabolic reactions, the estimated turnover rates were consistent across all four conditions (a total of 284 turnover rate values; Fig. 4a), with high correlation between any two conditions (Fig. 4b and Supplementary Fig. 5). The computed turnover rates were averaged across experimental conditions to give the largest set of flux-per-enzyme parameters estimated computationally to date under in vivo conditions. It is important to note that these estimated turnover rates do not have a direct relationship with fundamental enzyme kinetic parameters obtained in vitro but can be viewed as an in vivo data-driven estimate of the enzyme turnover rate.

Although these correlations provide information about relationships between biological components and, in some cases, take on predictive value (Fig. 5a), understanding their collective influence on cell physiology is harder to decipher. This issue can be addressed using a genome-scale model that assesses cost-benefit tradeoffs from a cell-centric perspective9,27. Genome-scale models (iOL1650-ME) compute the value of cellular components relative to the function of all other cellular components. To this end, the turnover rate values provide the minimum ‘capital expenditure’ for protein synthesis required to achieve a unit of flux through a given reaction. Thus, as a group, the calculated turnover rates provide coupling between proteome allocation and achievement of a physiological state.

The knowledge of the biological regularities identified in this work enables the parameterization of coupling constraints used in a genome-scale model of metabolism and gene expression metabolism and gene expression (ME). A parameterized model allows for prediction of responses to environmental perturbations. We tested the predictive capacity of a model containing parameter values derived from multiple conditions described above, to compute optimal cellular composition under new environmental conditions where proteomics data was not available. In simulations with our parametrized model, we perturbed a reference growth state through the addition of nutrients to the medium: batch growth on glucose was supplemented with adenine, glycine, tryptophan or threonine. From these predicted phenotypic states, we identified enzymes that were predicted to be differentially used in the supplemented condition (Fig. 5b,c). To validate these predictions, we collected omics data sets under these four perturbed conditions, to compare gene expression changes with the computated responses.

When validating our predictions using experimentally measured differential gene expression, we find our prediction accuracies of differential gene expression range between 56 and 100% (Table 1), and are significantly enriched for experimentally differential genes, with p-values ranging from 0.04 to 4 × 10−6 using a hypergeometric test (Fig. 5b). Therefore, using the parameterized model, we are able to improve the prediction of gene regulation that accompanies changes in growth environment. For example, a nutrient supplementation will often cause non-intuitive shifts in what precursors the cell uses to synthesize amino acid molecules. The parameterized ME model correctly identifies the production/non-production of L-serine from supplemented L-threonine and glycine (Fig. 5c and Supplementary Note 5).

## Discussion

The unprecedented growth in the type, size and complexity of biological data sets over the past couple of decades has led to a pressing grand challenge in biology referred to as BD2K. In this study, we address this critical need through the development of advanced data integration methods to enable multi-level analysis of genomic, transcriptomic, ribosomal profiling, proteomic and fluxomic data across multiple experimental conditions. We can show that pairwise integration of primary omics data reveal unknown biological regularities that quantitatively tie key cellular processes together, and that genome-scale models enable the quantitative synchronization of disparate omics data types, leading to the discovery of additional system-based novel regularities. For example, when directly compared, RNA and protein values have long been known to be poorly correlated16,28. However, the integration of ribosome profiling and structural data demonstrates how the secondary structures in proteins correlate with sites of translational pausing, supporting the theory of co-translational folding and the rhythm of translation29,30 (Supplementary Note 1). The variation in translation observed from multiple omics types sheds light on how the protein-per-RNA ratio is correlated for each gene across conditions, but poorly correlated across genes31.

By designing algorithms to integrate omic data with genome-scale networks, the ability to predict differential gene expression (Fig. 5c) emerges. On the most basic level, a genome-scale model of metabolism informs the user of all possible metabolic reactions and their respective stoichiometries8. The previous unparameterized ME model of E. coli19 added an additional reconstruction of gene and protein expression networks, and their associated metabolic costs. Here, we integrate omics data directly by using a parameterization algorithm to improve estimates of expression costs. We can demonstrate the increased accuracy of the parameterized ME model compared with the previous unparameterized ME model and M-model (Table 1 and Supplementary Fig. 6).

Taken together, we have shown that fundamental contextualization of multi-omic data leads to (i) insights into underlying biological mechanisms during protein translation and (ii) predictive computations based on cellular-econometric cost-benefit ratios associated with cellular functions. Thus, both multi-omic data analysis and genome-scale models will play an important role in establishing big data analysis frameworks to explain and predict cellular physiology.

## Methods

### Ribosome profiling

To compute the ribosome per protein ratios, E. coli MG1655 cells were grown in glucose (5 g l−1), pyruate (sodium pyruvate 3.3 g l−1), fumarate (disodium fumarate 2.8 g l−1) and acetate (sodium acetate, 3.5 g l−1). Ribosome profiling data sets were generated and analysed according to the following procedure, also detailed in Latif et al.13. Chloramphenicol (100 μg l−1) was added 2 min before harvest, cells were harvested at mid-log (OD600 0.4) by centrifugation at 5,000 g for 3 min. Ice-cold lysis buffer (25 mM Tris pH 8.0, 25 mM NH4Cl, 10 mM MgOAc, 0.8% Triton X-100, 100 U ml−1 RNase-free DNase I, 0.3 U μl−1 Superase-In, 1.55 mM chloramphenicol and 17 μΜ 5′-guanylyl imidodiphosphate) was added and the cells were resuspended quickly, followed by flash freezing in liquid nitrogen. Repeated freeze–thaw cycles were used to lyse cells followed by addition of sodium deoxycholate to a concentration of 0.3%. Lysate was then clarified by centrifugation. RNA (25 AU) was digested using 6,000 U of MNase for 2 h at 25 °C and quenched by adding 2.5 μl of 500 mM EGTA. Monosomes were recovered using Sephacryl S400 Microspin columns, followed by removal of ribosomal RNA using a Ribo-Zero-rRNA Removal Kit (Epicentre). The 3′-ends of the RNA fragments were desphosphorylated using T4 Polynucleotide Kinase for 30 min at 37 °C. The NEBNext Small RNA Library Prep Set for Illumina protocol was carried out till the 5′-adapter ligation step. Transfer RNAs were removed using hybridization to custom DNA oligo probes, followed by RNAse H treatment. The NEBNext protocol was then completed and sequencing was carried out on a Illumina MiSeq.

As the MiSeq did not provide sufficient read depth for the in-house ribosome profiling data sets to confidently determine pause locations, ribosome profiling data was obtained from Li et. al.11 for MOPS Rich and glucose MOPS minimal media (GEO Accession: GSE53767). Ribosome densities were derived using a similar protocol to Li et. al.11. Adapters were trimmed using cutadapt32 version 1.8. Reads were mapped using bowtie33 version 1.0.0 to E. coli MG1655 (NC000913), allowing for a maximum of one mismatch. Reads mapping to tRNA, ribosomal RNA and other non-coding RNA locations were discarded. There has been considerable discourse about the location of the A- and P-site relative to the ends of the reads, and not to bias our analysis based on the location we chose to assign reads to the 3′-end, which has been shown to be better conserved and aligned in prokaryotic ribosome profiling data sets34,35. Ribosome density across each gene was then dropoff corrected by fitting to an exponential function as was done in Li et. al.11.

### Genome-wide secondary structure annotations

The GEM-PRO reconstruction for E. coli iJO1366 (refs 17, 36) was used to provide structure-based annotations for the most representative protein structures found in the publicly available PDB database37. Protein data bank (PDB) files were parsed using STRIDE38 and Biopython39, to determine the location of secondary and tertiary structural elements on a codon-specific basis. This resulted in high-confidence secondary and tertiary structure annotations for 623 non-transport (or membrane-bound) genes in E. coli.

### Tertiary structure and protein domain annotations

Starting from the protein structures linked to metabolic genes in the GEM-PRO model, we annotated tertiary domains for each protein using the SCOP knowledgebase40 and FATCAT41 alignment tools. As a result of this analysis, the fraction of the protein aligning to an annotated tertiary domain was recorded and stored as an additional data type in the GEM-PRO reconstruction. The starting and ending amino acid of every domain within a protein were quality controlled and checked by aligning the PDB sequence with the amino acid sequence (FASTA) from E. coli MG1655 to fix offsets between the PDB residue numbering scheme and the actual amino acid sequence numbers. Hypergeometric enrichment testing was performed, to determine codons upstream and/or downstream from the start and end of any tertiary domain annotation that is enriched for pause sites.

### Identification of SD-like codons

Similar to those defined in Li et. al.20, we considered the following SD-like codons: 5′-AGG',GGA',GAG',GGG',GGT',GTG'-3′. Nucleotide sequences from E. coli MG1655 (Genbank accession: NC000913 (ref. 42)), were read in-frame to identify SD-like codon positions. Hypergeometric enrichment testing was used to determine downstream codons enriched for pause sites.

### Ribosome density and pause site accounting

The ribosome density (Supplementary Figs 2 and 3) at each codon was summed across all three nucleotides and divided by the mean of the gene, to get the normalized density. In an effort to increase the signal in an inherently noisy data type, pause sites were defined as codons, which had a normalized density of over 5, instead of on a per nucleotide basis as was done in Li et. al.20.

Hypergeometric enrichment testing was used to determine enrichment for pause sites at codon positions downstream from the ends of pause sites. P-values were calculated based on the formula for the survival function (1−cumulative distribution function) shown below:

where N refers to the total number of codons in the genes tested, m refers to the number of secondary structures, k refers to the total number of pause sites and x refers to the number of pause sites that fell on a specific codon position downstream of the secondary structure we are testing.

Codons downstream from α-helices, β-sheets and turn secondary structural elements, as well as SD-like sequences, were considered to be significantly enriched for pausing, if the hypergeometric enrichment tests indicated that the p-values <0.01. To calculate the number of pause sites accounted for by sequence and/or structural elements, pause sites that did not align with secondary structure or SD-like sequences were labelled ‘unaccounted’. The same procedure was used for determining the proportion of pause sites accounted for by structural features and SD-like sequence features.

### Computational method for predicting keff parameters

As noted above, ME simulations require several parameters, one of which is the effective catalytic rate of enzymes keff, which in turn affects the proteomic and ribosomal cost of running each reaction. Although using solvent accessible surface area (SASA) as a first approximation results in a correct overall prediction of 80% of the cell proteome by mass, improving these parameters can greatly affect the predictive power of the model for specific genes. We make use of the most extensive quantitative proteomic data in E. coli to date, which accounts for 55% of all open reading frames and 95% of the proteome14. Because of the difficulty in simultaneously predicting effective catalytic rates and reaction flux values, we developed an iterative workflow for updating the model parameters. This workflow is described below and each section corresponds to a panel in Fig. 3.

### Part A iterative simulation procedure

These ME simulations had the overall goal of minimizing the difference between the simulated proteome and the measured proteome for each experimental condition. The growth rate μ was set to the experimentally determined values (Supplementary Data 1). To improve solution times with the SoPlex linear programming solver43 with our formulation, we collapsed linear pathways into single reactions using cobrapy44, which were detected by identifying metabolites present in exactly two reactions.

To reconcile experimentally measured protein concentrations with the simulations, we want to solve a linear program, which will minimize the Manhattan distance between the expressed and measured protein production at the fixed growth rate. The Manhattan distance was used instead of the Euclidian distance, because it can be computed in the context of a linear program, whereas a Euclidian distance minimization requires a quadratic objective, which the SoPlex solver can not handle. To construct this problem, we added the following additional constraints to the ME linear program in terms of the corresponding measured protein amounts yi for each predicted gene translation flux variable xi (unmeasured proteins had no applied constraints).

This allowed us to minimize the error term , which is equivalent to minimizing and gives the closest flux state to the experimental data, while satisfying the ME constraints when solving the linear program. If these parameters resulted in an infeasible model, which could not simulate growth at μ, the simulation was halted. Otherwise, using the predicted fluxes predicted by this simulation and the experimentally measured proteomics data, we calculated the keff for each reaction enzyme, which we used in the next iteration of this workflow. This simulation loop was run a total of three times, to allow the loops to converge to a set of keff values.

### Part B sampling and simulation

The iterative simulation procedure described above might give a flux state, which is dependent on the original set of keff parameters used in the first round. Therefore, the keff were randomly initialized within two orders of magnitude of the value computed from SASA. The iterative sampling procedure was repeated repeated 300 times for each experimental condition and each time a new random keff parameter set was generated in the manner described.

### Part C result aggregation and filtering

For each experimental condition, the loop was started 300 times. However, as some parameter sets were unable to simulate growth at μ, some subset of those simulations failed before reaching three iterations. It was run successfully through 3 loops 148 times for glucose, 186 times for pyruvate, 97 times for fumarate and 83 times for acetate. Between these successful runs, there was a slight variation in reactions used because of the different starting keff parameters. Therefore, only reactions that were active for 90% of the successful runs were considered. These parameters were averaged to give a consensus set of keff parameters for each condition.

### Part D cross-condition parameter comparison

The intersection of these keff parameters under each condition was determined between all four conditions (Fig. 4a). The 284 parameters for reaction/catalyst pairs, which were in all conditions, were averaged to get a consensus set of keff parameters, which were used for ME computations. In addition, the pairs in common between each of the conditions were compared to give Pearson’s correlations, shown in the table in Fig. 4a.

### Predicting differential gene expression with iOL1650-ME

Simulations were performed using the same procedure as in the iOL1650-ME manuscript19 for batch growth on D-glucose with both keff parameter sets A and A+B. For each supplementation simulation, the uptake reaction for that particular metabolite was set to be unbounded. In the case of the adenine supplementation, the reactions HXAND, XAND and URIC were blocked (Supplementary Fig. 7). Genes that changed by more than a factor of 16 (a log2 change of more than 4) were predicted to be differentially expressed. This gives a stringent criterion, which identifies genes that are predicted to change by a significant-enough magnitude to manifest experimentally (by comparison, a log2 change of 2 is often used with microrray gene expression data to filter out changes in expression, which, although statistically significant, are not of a high-enough magnitude to really be considered relevant). Predictions of gene differential expression were considered correct if cufflinks obtained a false discovery rate of <0.05 for that gene in the mRNA sequencing data and the gene expression changed in the same direction (either both increase or both decrease) in both the predictions and mRNA sequencing data. Hypergeometric enrichment p-values were calculated using the scipy statistics package using the survival function + ½ × probability mass function of the distribution.

### Data availability

RNA sequencing data generated in this study is available from the NCBI Gene Expression Omnibus (GEO) under accession numbers GSE59759 and GSE59760. Measured growth rates are available in Supplementary Data 1 and estimated keff parameters in units of per second are available as Supplementary Data 2. All other relevant data are available from the authors upon request.

How to cite this article: Ebrahim, A. et al. Multi-omic data integration enables discovery of hidden biological regularities. Nat. Commun. 7, 13091 doi: 10.1038/ncomms13091 (2016).

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## Acknowledgements

This work was funded from a generous gift from the Novo Nordisk Foundation to the Center for Biosustainability (grant NNF16CC0021858). It was also supported by DE-FOA-000014 from the U.S. Department of Energy, NIH R01 GM057089 from the National Institutes of Health and by the Swiss National Science Foundation (grant p2elp2_148961 to E.B). This research used resources of the National Energy Research Scientific Computing Center, which is supported by the Office of Science of the US Department of Energy under Contract Number DE-AC02-05CH11231. We gratefully acknowledge Dr Mahmoud Al-Bassam and Dr Jinwoo Kim for scientific discussions on ribosome profiling.

## Author information

Authors

### Contributions

Conceptualization, A.E., E.B., J.T. and B.O.P. Methodology (ME modelling: A.E., J.L., E.J.O. and A.M.F.; ribosome profiling analysis: E.B., J.T.). Investigation, J.T., A.E. and E.B. Writing original draft, E.B., A.E. and B.O.P. Writing, review and editing, E.B., A.E., J.T., J.L., A.M.F. and B.O.P. Discussion, all authors. Funding acquisition, resources and supervision, B.O.P.

### Corresponding author

Correspondence to Bernhard O. Palsson.

## Ethics declarations

### Competing interests

The authors declare no competing financial interests.

## Supplementary information

### Supplementary Information

Supplementary Figures 1-9, Supplementary Notes 1-5, Supplementary Methods and Supplementary References (PDF 1540 kb)

### Supplementary Data 1

Measured growth rates on Glucose, Acetate, Fumarate, and Pyruvate (XLSX 202 kb)

### Supplementary Data 2

Computed model keff parameters (CSV 108 kb)

## Rights and permissions

Reprints and Permissions

Ebrahim, A., Brunk, E., Tan, J. et al. Multi-omic data integration enables discovery of hidden biological regularities. Nat Commun 7, 13091 (2016). https://doi.org/10.1038/ncomms13091

• Accepted:

• Published:

• DOI: https://doi.org/10.1038/ncomms13091

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