Figure 4: Material transfer is not the result of nuclear fusion. | Nature Communications

Figure 4: Material transfer is not the result of nuclear fusion.

From: Donor and host photoreceptors engage in material transfer following transplantation of post-mitotic photoreceptor precursors

Figure 4

(ac), representative confocal images of retinal sections from Gnat1−/− and wild-type retinae following transplantation of NrlGFP post-mitotic photoreceptor precursors (green), showing expression of Lamin B (red). GFP+ cells (arrows) within the host ONL only ever presented a single nuclear envelope (red) and nucleus (labelled with Hoechst; blue). Right hand panels show nuclei and Lamin B staining only. Scale bars 10 μm (dg), FISH for the male Y-chromosome. (d), Male and (e), female NrlGFP (green) retinal sections stained for the Y-chromosome (red), which appears as a red dot. Insert, Male GFP+ cells with Y-chromosome staining. No Y-chromosome staining was seen in the female sections (e). (f,g), examples of GFP+ cells within female host retinae 5-6 weeks post-transplantation of male NrlGFP donor cells, showing a GFP+ cell that was positive for Y-chromosome staining (f, insert) and another that was GFP+/Y-chromosome (g, insert). Right hand panels show GFP and Y-chomosome staining only. Scale bars 25 μm

Back to article page