Figure 5: Comparison of vesicle intensity extraction done by 2D flow nanometry and NTA. | Nature Communications

Figure 5: Comparison of vesicle intensity extraction done by 2D flow nanometry and NTA.

From: Two-dimensional flow nanometry of biological nanoparticles for accurate determination of their size and emission intensity

Figure 5

a and b show representative intensity traces for a single, fluorescently labelled vesicles, while c and d compare the intensity-versus-size parameter plots obtained by NTA (grey dots) and 2D flow nanometry (black dots) for two different batches of SUVs (created by sonication (c) and extrusion (d) as described in the ‘Methods’ section). Both batches show peak hydrodynamic radii around 38 nm, but differ in their polydispersity. Due to much lower intensity fluctuations observed in 2D flow nanometry (a) with respect to NTA (b), the expected scaling law is well visible in the parameter plots (solid lines in c,d), while it is hard to resolve for NTA data.

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