Figure 3: CHX and other eukaryote-specific but not prokaryote-specific antibiotics display anti-leukaemic activity. | Nature Communications

Figure 3: CHX and other eukaryote-specific but not prokaryote-specific antibiotics display anti-leukaemic activity.

From: Structure–function insights reveal the human ribosome as a cancer target for antibiotics

Figure 3

(a,b) Eukaryote-specific antibiotics: anisomycin (ANS), cycloheximide (CHX), deoxynivalenol (DON), homoharringtonine (HHT), verrucarin-A (VA); prokaryote-specific antibiotics: gentamicin (GEN), kanamycin (KAN), streptomycin (STR), tetracycline (TCN). Top: WST1 assay of mitochondrial activity and cell survival, 48 h after incubation of KO99L tPTEN−/− cells with increasing doses of the different compounds. Middle: flow cytometry analysis of cellular counts, 48 h after incubation of KO99L cells with indicated molecules. The results are representative of at least three independent experiments. Bottom: flow cytometry analysis of cell death induction by the different compounds, 48 h after incubation of KO99L cells. Data represent live cell numbers (4,6 diamidino-2-phenylindole (DAPI) negative cells). (c) Top: WST1 assay of mitochondrial activity and cell survival of human cell lines representative of T-ALL (Jurkat), B-ALL (NALM-6, 697) and AML (Kasumi-1, U937). Assays were performed after a 48 h incubation with increasing doses of the different E-specific antibiotics. Bottom: flow cytometry analysis of cell death induction by the different E-specific antibiotics (48 h incubation) on the leukaemic cell line panel. (d) Immunoblotting of murine c-myc, Mcl-1, Hsp-90 proteins and of mitochondrial Cox-2 after incubation of tPTEN−/− KO99L cells for 24 h with indicated doses of the different antibiotics. The results are representative of at least three independent experiments.

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