'Marine biofouling', the undesired growth of marine organisms such as microorganisms, barnacles and seaweeds on submerged surfaces, is a global problem for maritime industries, with both economic and environmental penalties. The primary strategy for combating marine fouling is to use biocide-containing paints, but environmental concerns and legislation are driving science and technology towards non-biocidal solutions based solely on physico-chemical and materials properties of coatings. Advances in nanotechnology and polymer science, and the development of novel surface designs 'bioinspired' by nature, are expected to have a significant impact on the development of a new generation of environmentally friendly marine coatings.
'arine biofouling, the colonization of submerged surfaces by unwanted marine organisms (Fig. 1), has detrimental effects on shipping and leisure vessels, heat exchangers, oceanographic sensors and aquaculture systems. For example, it has been shown that the increased roughness presented by a heavily fouled ship hull can result in powering penalties of up to 86% at cruising speed; even relatively light fouling by diatom 'slimes' can generate a 10–16% penalty1. Without effective antifouling (AF) measures, in order to maintain speed, fuel consumption (and therefore greenhouse gas emissions2) increase significantly. A recent analysis of the economic impact of biofouling for the Arleigh Burke DDG-51 destroyers, which comprise 30% of the ships in the US Navy fleet, estimates the overall cost associated with hull fouling at $56 million per annum3. This figure is based on the present AF coating system, cleaning and fouling level (typically heavy slime) of the Navy. If the analysis is extended to the entire US Navy fleet, the approximate cost of hull fouling is between $180 and 260 million per annum.
Marine biofouling is ubiquitous and has been a practical problem ever since man sailed the oceans; controlling it, without simultaneously creating unacceptable environmental impacts on non-target species is a considerable challenge. Recent years have seen a resurgence of interest in the fundamental science behind the processes involved in biofouling, and in the design of novel coatings and other non-coating technologies. The main driver for this is legislation that has outlawed some highly effective AF paints, notably the use of tributyltin oxide, and posed a stricter evaluation and regulatory regime on the use of alternative biocides. 'Green' alternatives to biocide-based technologies are therefore urgently sought by the marine coatings industry, and there is considerable interest in developing biocide-free coatings that rely on surface physico-chemical and bulk materials properties to either deter organisms from attaching in the first place ('prevention is better than cure') or reduce the adhesion strength of those that do attach, so that they are easily removed by the shear forces generated by ship movement or mild mechanical cleaning devices.
Fouling-resistant coatings for application to large-scale structures, such as ship hulls, are based on polymeric 'binders'—the film-forming component of paint. Advances in macromolecular synthesis, self-assembling polymers, hyperbranched and interpenetrating polymer networks and various types of nanocomposites, provide opportunities for developing novel binder systems. Natural AF surfaces also provide a source of inspiration for new coating designs.
This review is aimed at the intersection between biology, materials sciences and engineering. Biological aspects of fouling and the particular challenges posed to the materials scientist confronted by the vast biodiversity in fouling species and the range of attachment behaviours and adhesion mechanisms adopted will first be considered. Some of the novel approaches currently being explored by materials scientists in developing coatings for marine applications, both from a fundamental and more practical perspective, will then be discussed. Emphasis will be given to interdisciplinary studies in which the structure and surface properties of coatings are correlated with their biological performance or in which predictive models are being developed that will facilitate rational design of coatings in the future.
Fouling organisms and their settlement and adhesion strategies
When a clean surface is immersed in natural seawater, it immediately starts to adsorb a molecular 'conditioning' film primarily consisting of dissolved organic material4. Colonization of the conditioned surface by a wide range of organisms depends on the availability of the colonizing stages, and their relative rates of attachment and surface exploitation. Fouling is a highly dynamic process; the specific organisms that develop in a fouling community depend on the substratum, geographical location, the season and factors such as competition and predation.
A distinction is often made among 'microfouling' (often referred to as 'slime') due to unicellular microorganisms such as bacteria, diatoms and protozoa, which form a complex biofilm; 'soft macrofouling' comprising macroscopically visible algae (seaweeds) and invertebrates such as soft corals, sponges, anemones, tunicates and hydroids; and 'hard macrofouling' from shelled invertebrates such as barnacles, mussels and tubeworms (Fig. 2).
It is often stated that surface colonization follows a linear 'successional' model5,6,7,8,9 in which bacterial biofilm formation is followed within a week by spores of macroalgae (seaweeds), fungi and protozoa, followed in turn, within several weeks, by larvae of invertebrates, such as barnacles. In reality, this 'classical' view is a considerable oversimplification as motile spores of seaweeds are capable of settling within minutes of presenting a clean surface10 and larvae of some species of barnacles, bryozoans and hydroids settle within a few hours of immersion11. For both algae and larvae, this is well within the 'one-to-several weeks' quoted by Wahl6. Rather than the linear successional model, the 'dynamic' model12,13 provides a more balanced view.
It is also misleading to assume that successional colonization of a surface necessarily implies a causal relationship between one stage and the next and even more misleading to assume that controlling or blocking initial stages of colonization, such as biofilm formation, will reduce or eliminate macrofouling. On the other hand, it is undoubtedly clear that attachment of spores and larvae can be influenced by other organisms, notably by bacterial biofilms, and positive, negative and neutral effects have been detected in controlled laboratory experiments when biofilms of specific bacteria have been tested against algal spores and larvae of invertebrates14,15,16,17,18. Some of these effects have also been reported in field observations16. However, colonization models are mostly based on studies of natural substrata, such as wood and stone or traditional AF coatings that rapidly foul, and there are only a few reports that study the colonization of modern, non-biocidal AF coatings by microorganisms19,20. Fouling by biofilms composed of communities of bacteria, diatoms, fungi and protozoa has been largely ignored until recently, because the focus of research has been on macrofouling. It is likely that more attention will be paid to slime fouling in future, now that its economic impact is firmly established1,3.
A major challenge in creating an effective fouling-resistant coating is that the diversity of fouling organisms is vast and the range of adhesion mechanisms (including adhesives) used is correspondingly great (Fig. 2). The colonizing ('recruitment') stages of fouling organisms range in size from micrometres (bacteria, single-celled spores of algae and some diatom cells) to hundreds of micrometres or even millimetres (larvae of invertebrates). Considerations of size are relevant to attempts (see Future Directions) to engineer surface topographies that may deter the settlement of organisms. However, the critical length scale in determining settlement is not necessarily the size of the organism per se, but rather the size of the parts or structures involved in the sensing apparatus of an organism (for example, the paired sensory antennules of barnacle cypris larvae21 (Fig. 2)), which determine whether the organism selects a surface for attachment.
Assuming that the organism has settled, the success of that organism in colonizing a surface and growing into a reproductive adult, within a turbulent marine environment, depends on how well the macromolecular adhesive polymers secreted by the settled organisms secure to the adhesive interface, which is determined by the interfacial molecular interactions that are in turn influenced by the properties of a surface or coating at the molecular or nanoscale level. Non-biocidal, fouling-resistant coatings are based on polymers designed to minimize molecular adhesive forces between the adhesives used by marine organisms and the coating. Rational design of coatings ultimately requires an elucidation of the chemical and physical interactions between the bioadhesives used by marine organisms and the prospective fouling-release polymer(s). Unfortunately, so few marine bioadhesives have been isolated and characterized that obtaining information at this level has proved to be difficult (see ref. 22 for comprehensive reviews of bioadhesives). An exception to this is the well-characterized marine mussel foot adhesive system based on byssal threads and adhesive plaques formed from a composite of several proteins23. Catechol groups of the amino acid DOPA (dihydroxyphenylalanine) are oxidized to react with other catechol groups to crosslink the proteins into a strong cohesive composite. Dihydroxyphenylalanine groups also hydrogen bond to metal ions in the mineral substrata. Surface-sensitive spectroscopic techniques have been used to understand molecular interactions between mussel adhesive proteins and various interfaces in situ. For example, sum frequency generation vibrational spectroscopy (SFG) has shown how substrate surface chemistry influences the structure of the adsorbed adhesive plaque protein Mefp-324. On a hydrophobic, non-polar substrate, to which Mefp-3 attaches weakly, strong SFG signals were detected in both C–H stretching and amide I regions, indicating that the adsorbed protein was in an ordered conformation. On a polar, hydrophilic polymer substrate to which Mefp-3 strongly adheres, no SFG signals in these regions were detected, showing that the protein adopted a more or less random or disordered structure. Application of this type of analysis to films based on polymers used in fouling-release coatings will prove instructive; however, until more marine adhesives of important fouling organisms are biochemically characterized and made available in purified form and in sufficient quantity for experimental purposes, the biophysical analysis of molecular events at adhesive/coating interfaces will have only limited impact on the design of novel coatings.
Insights from in situ imaging of surface exploration
One approach to the development of novel AF coatings is to create a 'deterrent' surface that inhibits the initial attachment of the recruitment ('settling') stages of spores, larvae and so on. These settling stages are 'choosy' in the sense that they exhibit behaviours that result in a surface being accepted or rejected for settlement. Although much can be done to assess such behavioural traits through simple assays of the ability of larvae or spores to settle on a surface, a more comprehensive understanding requires detailed observation of the behaviour of organisms as they explore the interface. In this section, we outline two current imaging technologies that are being used to enhance our understanding of organism behaviour in relation to specific surface properties. This information will provide improved insight into the chemistry and thermodynamics of adhesion and will help to formulate the 'design rules' by which novel materials may be developed to deter settlement.
Two-dimensional tracking of the cypris larvae of barnacles has been studied using EthoVision tracking software25. Aldred et al.26 explored cyprid behaviour on two zwitterionic polymer coatings (poly(sulfobetaine methacrylate) (polySBMA) and poly(carboxybetaine methacrylate) (polyCBMA)), both of which completely inhibited cyprid settlement and are resistant to protein adsorption27. On polySBMA and on bare glass, cyprids explored the surface, but were unwilling or unable to settle (Fig. 3), whereas on polyCBMA, cyprids did not attempt exploration and left the surface quickly.
Although tracking the behaviour of fouling organisms aids in understanding surface selection, a more detailed understanding of interfacial interactions also requires information at the molecular level. Imaging surface plasmon resonance (iSPR) has been applied to in situ, real-time analysis of temporary adhesive deposition by barnacle larvae as they explore a surface28. Footprints of temporary adhesive were imaged on a bare gold-coated surface and a self-assembled monolayer of oliogoethylene glycol, selected for its well-known resistance to protein adsorption. Larvae were observed to probe both surfaces with their sensory structures, but only on the bare gold surface did the point of contacts leave deposits of temporary adhesive (Fig. 4). The frequency with which the secreted protein sticks to the surface provides a proxy indication of the strength of the protein-surface bond28. This example demonstrates the power of iSPR to generate novel information on the exploration of different surfaces at the molecular level; what is now needed is to apply this approach to more complex, candidate experimental coatings for marine application, rather than simple model substrates, such as OEGs (oligo(ethyleneglycol))—this may require further technical improvements to iSPR.
Novel non-biocidal coating strategies
Two general (non-exclusive) strategies are typically followed in the design of novel, non-biocidal, non-fouling surfaces. In this review, we distinguish between AF coatings, in which the objective is to deter the recruitment stages of fouling organisms from attaching in the first place, and 'fouling-release' (FR) coatings, which do not prevent organisms from attaching, but the interfacial bond is weakened so that attached organisms are more easily removed by the hydrodynamic shear forces generated by movement of the ship through the water29 or by gentle 'grooming' devices30. In both cases the objective is to achieve the desired result through manipulation of the physico-chemical and/or materials properties of the coating (for example, elastic modulus, frictional coefficient) so that the organism either perceives the surface as unconducive to settlement or the intermolecular interaction forces between the surface and the polymeric adhesives produced by the fouling organism are weakened, promoting adhesive failure. These two general approaches are not mutually exclusive and in fact the distinction is overly simplistic.
Most current commercial FR coatings are based on poly(dimethylsiloxane) elastomers (PDMSe)31. These non-polar, low surface energy (∼22 mN m−1), hydrophobic polymers would be expected to show low adhesion of polar molecules (including adhesive proteins) because of reduced opportunities for H-binding and polar interactions. Coatings based on PDMSe also have typically low elastic moduli, and the release of hard-fouling is proportional to (γE)½; where γ is the surface energy and E is the modulus32. Although PDMSe exhibit the desired combination of low surface energy (to minimize the work of adhesion) and low modulus, such coatings suffer from some disadvantages. Because of their low surface energy, they are difficult to bond to a substrate without an appropriate tie coat. They are less durable, more easily damaged than other types of coating and they frequently 'fail' to brown slimes dominated by diatoms that attach more strongly to hydrophobic surfaces19,33. The fouling-release technology is also most effective when applied to high-activity, fast-moving (>15 knot) vessels and is less suitable for vessels that spend long periods in port or which cruise at lower speeds to maintain fuel efficiency. For these and other reasons, there has been extensive research on newer fouling-release technologies31, which may either 'toughen up' silicones through the use of new tie-coats and the incorporation of fluoropolymers or which seek to create a surface complexity. Currently in vogue are amphiphilic coatings, which incorporate some of the benefits of both hydrophobic and hydrophilic functionalities, for example, the latest fouling-release coating from International Paint, Intersleek 900. Such coatings, through phase separation, for example, of mutually incompatible block copolymers, create a dynamic surface with local variations in surface chemistry, topography and mechanical properties. The general aim is to create a dynamic and compositional surface complexity that deters settlement stages of fouling organisms and reduces the interfacial bonding with adhesive polymers.
Here we review the range of experimental coating technologies currently being explored as the basis for future practical AF coatings. Only a few of these technologies have reached the stage of evaluation in the field, for example, as test panels on rafts, and there are no published reports of field performance. The main focus will be on polymer-based experimental systems that can be envisaged as the basis for future practical coatings for application to ship hulls. The accent will be on studies that demonstrate clear coating structure/property/performance correlations and the importance of using surface characterization techniques under in situ (that is, immersed) conditions in order to assess surface reorganization and to understand the type of surface that the settling stages (cells and larvae) encounter. The section will also consider the current interest in biomimetic/bioinspired technologies and efforts to rationalize the influence of surface roughness through predictive models.
Bioinspired engineered topographies
It is often observed that many marine organisms do not become colonized by other species8,34,35,36,37. A diverse range of mechanisms has been implicated in natural defence, including settlement-inhibiting micro- and nanotopographies, secreted bioactive molecules, sloughing surface layers, mucus secretions and hydrolytic enzymes (see ref. 38 for review). Natural mechanisms may be used as the basis for 'biomimetic' or 'bioinspired' coatings, and most attention has been devoted to designs based on topographical features. The surfaces of many marine animals ranging from shells of molluscs to the skin of sharks and whales have a complex surface topography, and by analogy with the 'self-cleaning' lotus-leaf effect, it is often speculated that this surface roughness may have a role in either deterring fouling organisms from attaching or promoting their easy release. These thoughts have encouraged research on a number of bioinspired surface designs35 of which the most prominent for marine applications are those that mimic sharkskin39 and invertebrate shells40.
Moulded topographies in PDMSe (Box 1), inspired by the skin of fast-moving sharks at ∼1/25th of the scale (Sharklet AF, Fig. 5), resulted in an 85% reduction in settlement of zoospores (motile spores) of the macroalga Ulva compared with smooth PDMSe39. The Sharklet AF topography consists of 2μm wide rectangular-like (ribs) periodic features (4, 8, 12 and 16 μm in length) spaced 2 μm apart. Further studies based on 2μm features correlated zoospore settlement with an empirically derived 'engineered roughness index' (ERII) for various surface designs41,42. ERII is a dimensionless ratio involving several geometric parameters of the surfaces:
where r is the Wenzel's roughness factor, (1−ϕs) is the area fraction of feature tops (that is, the ratio of the depressed surface area between features and the projected planar surface area) and df is the degree of freedom of spore movement (1 or 2). Spore settlement decreased with an increase in ERII. Sharklet AF with an ERII of 9.5 showing a 77% reduction in settlement compared with the smooth surface.
Schumacher et al.43 introduced the concept of nanoforce gradients to explain how different feature geometries based on the Sharklet design might exert an influence on the settlement of spores. It was hypothesized that nanoforce gradients caused by variations in topographical feature geometry will induce stress gradients within the lateral plane of the cell membrane of a settling cell during initial contact. Perception of such stress gradients by presumed mechanotransducer proteins in the cell membrane could, through intracellular signal cascades, lead to modification of the settlement response. The generation of the nanoforce gradients was envisaged as a function of the bending moment or stiffness of the topographical features with which the cell is in contact. The geometric dimensions, including width, length and height of the topographical feature, as well as the modulus of the base material, define its stiffness. By introducing geometric variations in features contained in the engineered topography, an effective force gradient between neighbouring topographical features is developed. To test the nanoforce gradient hypothesis, modifications were made to the design of Sharklet AF, resulting in a range of nanoforce gradients43. The surfaces were then challenged with spores of Ulva. Surfaces with nanoforce gradients ranging from 125 to 374 nN all significantly reduced spore settlement relative to a smooth substrate, but the level of inhibition did not correlate directly with the magnitude of the gradient, nor was the level of inhibition predicted by ERII. It became evident that the number of distinct features was a better predictor of inhibition by the topographies and this led to substitution of df by n, in a revised ERI model (ERIII).
Settlement densities of spores of Ulva were affected by the complexity of the microtopographical patterns, settlement decreasing as the number of features increased (Fig. 6). The number of attached spores per unit area was normalized to the number of spores attached to a smooth control and the data were transformed by taking the natural logarithm (ln A/Ao). The spore settlement density on the different patterns correlated well with the number of features and the ERI (ERIII) model42. Furthermore, the level of spore settlement on several novel surface designs and some designs tested previously was correctly predicted by the ERIII model42.
These results confirm that the designed nanoforce gradients may be an effective tool and predictive model for the design of unique non-toxic, non-fouling surfaces for marine applications. However, as different fouling organisms respond to topographies of different length scales, hierarchical patterning may be required. An initial study by Schumacher et al.44 indicated that complex designs would be required to repel multiple settling species. Current research on the 'Sharklet effect' attempts to combine topography with different chemistries at the surface or in the bulk, to further intensify the deterrent effect. It should also be pointed out that to be successful, bioinspired technologies will require multiple attributes (topography, modulus and chemistry) to be effective in the marine environment. The organisms that have provided the inspiration for this area of research, for example, mussels, use several strategies to keep themselves 'clean'34, and hierarchical topographic designs will be needed to combat settlement of all cells and larvae44.
Amphiphilic nanostructured coatings
A recent trend in designing experimental coatings for AF/fouling-release purposes has been to create surfaces with compositional (chemical) heterogeneity at the nanoscale through the thermodynamically driven, phase segregation of polymer assemblies, followed by crosslinking in situ. These coating designs may be based on blends of immiscible polymers or contrasting chemistries of block copolymers, and the general aim is to combine the non-polar, low surface energy properties of a hydrophobic (typically fluorinated) component to reduce polar and hydrogen-bonding interactions with the bioadhesives used by fouling organisms, with the well-known protein repellency properties of the hydrophilic components, typically, oligo(poly)ethylene glycols. The resulting chemical ambiguity, expressed in terms of amphiphilic nanodomains on the surface (Fig. 7), may lower the entropic and enthalpic driving forces for the adsorption of the marine protein and glycoprotein bioadhesives, which are themselves amphiphilic in character45,46. Lau et al.47 have speculated that the often-observed protein resistance of some nanopatterned, amphiphilic diblock copolymers is due to the intrinsic high density of surface interfacial boundaries.
The first published example of an amphiphilic coating for use in marine AF is that of ref. 48, based on hyperbranched fluoropolymers and linear poly(ethylene glycols) (PEG), which self-assemble on cross-linking to form complex surface topographies and chemical domains of both nanoscopic and microscopic dimensions. The surface patterns were strongly influenced by immersion and by the relative proportions of the two polymers. The coating design anticipated that this surface complexity would either have a deterrent effect on the settling stages of fouling organisms or would be unfavourable for adsorption and unfolding of adhesive proteins. Gudipati et al.49 subsequently showed that several macromolecules of biological origin (proteins and lipopolysaccharides) showed reduced adsorption to compositions with high concentrations of PEG (45–55% by weight). These compositions were also effective against settlement of zoospores of the green alga Ulva, and small plants of Ulva adhered less well to some of the compositions compared with PDMSe.
Krishnan et al.50 used side-chain grafting of hydrophilic oligoethylene glycol and hydrophobic perfluoroalkyl side chains to a preformed poly(styrene-block-acrylic acid) copolymer. Martinelli et al.51,52 developed similar amphiphilic copolymers using controlled atom transfer radical polymerization and a purely polystyrene backbone. In both cases, these surface-active block copolymers were deposited as a thin film over a thick layer of SEBS (poly(styrene-block-ethylene-random-butylene)-block-polystyrene), an elastomeric thermoplastic material that provides a suitable low modulus53. In both studies, NEXAFS and angle-resolved X-ray photoelectron spectroscopy measurements demonstrated that the film surface underwent reconstruction resulting from the thermodynamically induced phase segregation of the mutually incompatible components. However, these are in vacuo techniques and it is difficult to translate the information they provide on surface structure in the dry state to the situation when these coatings are immersed in water. To understand the relationship between surface properties and biological performance, it is necessary to perform surface characterization in the immersed state. In situ (underwater) atomic force microscopy showed that surface topography changed on immersion, presumably caused by localized swelling of the hydrophilic blocks51 (Fig. 8). Information on the surface chemistry of immersed coatings was provided by Sum Frequency Generation spectroscopy, which showed that, in water, the hydrophilic PEG segments migrated to the surface and surprisingly the hydrophobic, fluorinated groups were still evident, suggesting that the surface is truly amphiphilic in nature54. These coatings have been tested in bioassays to explore their intrinsic ability to resist the settlement and reduce the adhesion strength of two marine algae, namely, the macroalga (seaweed) Ulva and the unicellular diatom Navicula. These organisms were chosen specifically because they show opposite preferences, Ulva tends to adhere most strongly to hydrophilic coatings while diatom species such as Navicula adhere most strongly to hydrophobic coatings, especially those that are silicone-based. Both Ulva and Navicula showed weak adhesion to the amphiphilic surface-active block copolymers coatings compared with a standard fouling-release coating of PDMSe46,51.
Phase-segregating siloxane–polyurethane copolymers
In an attempt to improve the mechanical properties of PDMS elastomers, thermosetting, cross-linked PDMSe–polyurethane nanohybrid copolymer coatings have been synthesized55,56,57,58. Certain compositions (those containing only 10% PDMSe) spontaneously phase-separated to form microtopographic domains composed of PDMSe surrounded by the polyurethane matrix. The domains appeared to increase in size after 2-week immersion: stability depended on the casting solvent55 and mixing time57 used. Compositions with a higher proportion of PDMSe did not form domains, but rather formed a smooth stratified coating of low surface energy PDMSe over a sub-layer of higher surface energy polyurethane. In detachment studies with 'pseudobarnacles' (an epoxy-bonded stud used as a proxy for live barnacles) and with reattached live barnacles, it was shown that the pull-off force in both cases was lower on compositions with surface domains than those without57.
Majumdar et al.59 investigated the AF and fouling-release properties of PDMSe with tethered quaternary ammonium salts (QAS). The initial concept was to combine the biocidal properties of QAS with the fouling-release properties of PDMSe. Surface analysis of the resultant coatings revealed a heterogeneous, two-phase morphology with increased water contact angle (increased hydrophobicity). Depending on the QAS alkyl chain length, surface protrusions were either isolated, 0.7–2.6 μm in size (C14), or inter-connected, 0.60–2.9 μm in size (C18). The C18 coatings also showed higher levels of nanoroughness. In laboratory tests with a range of fouling organisms, the rougher C18 coatings showed the best fouling-release performance against the macroalga Ulva exceeding that of a commercially available FR coating. FR performance against Ulva is unlikely to be related to the tethered biocidal functionality per se, as the coatings did not affect growth of the alga, but rather seems to be related to the nanoroughness. On the other hand, it was shown that the QAS coatings inhibited microbial biofilm formation of the bacterium Cellulophaga lytica and the diatom Navicula incerta—which is most likely due to the biocidal functionality.
Superhydrophilic zwitterionic polymers
The limitations in terms of stability of protein-resistant molecules such as PEG for marine applications have been referred to earlier. A more promising group of chemistries that deter the adsorption of proteins and cells are zwitterionic materials, such as poly(sulphobetaine) and poly(carboxybetaine). These materials have good chemical stability and low cost60. The majority of the published papers on zwitterionic coatings relate to proteins, serum or medically important bacteria (reviewed in ref. 60). In AF assays polySBMA brushes grafted onto glass surfaces through surface-initiated atom transfer radical polymerization almost completely inhibited the settlement of spores of the green alga Ulva and the attachment of diatom cells was also strongly reduced. In both cases, the few cells that did settle were only loosely attached61. Both polySBMA and polyCBMA resisted settlement of barnacle cypris larvae, but as noted above (Insights from in situ imaging of surface exploration), larval searching behaviour differed on the two zwitterionic surfaces26. The resistance of zwitterionic materials to the adsorption of proteins and cells is generally attributed to a strong electrostatically induced hydration layer that creates a superhydrophilic surface. In the case of marine organisms this means that the secreted proteoglycan bioadhesives are unable to achieve a strong interfacial bond by excluding water molecules from the interface. The future development of hydrolyzable zwitterionic esters as coatings should provide a platform for the development of practical marine coatings60.
Organically modified, hybrid xerogel coatings prepared by the sol-gel process have been shown to possess AF and fouling-release characteristics62,63,64,65. The xerogel surfaces are inexpensive and robust, characterized by uniform surface roughness/topography and cover a range of wettabilities and surface energies. The settlement of barnacle cyprids and algal zoospores was highly correlated with surface energy and wettability, as was the removal of algal (Ulva) sporelings (young plants) and adhered diatoms62,65. However, the response to these surface parameters was not uniform; cypris larvae of Balanus amphitrite prefered to settle on xerogel surfaces with high wettability and high surface energy65, while zoospores of Ulva prefered to settle on hydrophobic xerogel surfaces with low surface energy62. Sporelings of Ulva were more readily removed from surfaces with low wettability and low surface energy (Bennett et al.62), while diatoms were more readily removed from surfaces with high wettability and high surface energy65. The current xerogel formulations are only suitable for low-fouling freshwater environments in which slime predominates, as the thin (∼1 μm) xerogels do not have fouling-release properties for adult barnacles. To improve AF performance in marine environments, sequestration of catalysts, for example, diorganoselenoxides and diorganotellurides, into the xerogel films that facilitate the oxidation of halide salts with naturally occurring hydrogen peroxide to form the corresponding hypochlorous acid have shown promise against the settlement of barnacle and tubeworm larvae and algal spores64.
Nanocomposites and superhydrophic surfaces
Hydrosilation-cured silicone elastomers, reinforced through the incorporation of small quantities of multiwall carbon nanotubes (MWCNTs) and sepiolite nanoclays, showed enhanced fouling-release performance in laboratory assays with algae and barnacles compared with unfilled PDMSe controls66. The use of MWCNTs is particularly significant, as the improved performance was obtained at low loadings of the nanofiller (0.05–0.2% by weight). At this level of loading the bulk properties of the coatings (tensile modulus and cross-link density) appear to be unchanged, but the coatings became slightly more hydrophobic and there was a significant change in shear-thinning behaviour67. The changed rheological properties were attributed, on both theoretical and experimental grounds, to a strong molecular affinity between the siloxane chains and the MWCNTs, through CH-π interactions involving methyl groups of the PDMSe and aromatic rings of the MWCNTs67. This affinity is also important in reducing the likelihood of MWCNTs being released into the marine (or any other) environment, which is relevant to the current debate on potential issues of nanoparticle toxicity. The improved fouling-release performance appears to be correlated with the effect of MWCNTs in inducing time- and immersion-dependent changes in surface nanotopography68. Under tapping-mode AFM, both unfilled and MWCNT-filled PDMSe showed a smooth morphology (root mean squared (RMS) roughness ∼1 nm), but after immersion in water, there was a complex time-dependent surface restructuring of both filled and unfilled coatings, and those containing 0.1% MWCNTs exhibited a significant restructuring at the nanoscale. It is not yet clear how such nanostructuring contributes towards fouling-release performance.
Superhydrophobic surfaces are water-repellent, having a high water contact angle (typically >150°) and a very low roll-off angle, that is, the inclination angle at which a water drop rolls off the surfaces69. The superhydrophobic effect relies on the trapping of air, as exemplified by a number of natural surfaces, for example, the lotus leaf and insect wings37. Recently, Scardino et al.36 reported three superhydrophobic coatings (SHC) that inhibit the settlement/attachment of spores of algae and larvae of several invertebrates. The coatings were made by spraying fumed silica-filled siloxanes. All three coatings were superhydrophobic (SHC 1 contact angle θA=169°, SHC 2 θA=155° and SHC 3 θA=169°) and exhibited either micro/nanoroughness (SHC 1 and 2) or only nanoroughness (SHC 3). All test organisms avoided settling on the SHC 3 coating, which had large pores of ∼350 nm in diameter and small pores in the range of 10–50 nm. Small-angle X-ray scattering was used to characterize the partial wetting of the superhydrophobic surfaces in situ under immersion conditions. The broad-spectrum AF effect was attributed to the larger amount of unwetted interface on SHC 3 compared with SHC 1 and 2 when immersed in seawater. Whether the unwetted interface will resist wetting (and hence the accumulation of fouling) following long-term immersion needs to be determined.
Current commercial, non-biocidal fouling-release coatings, suffer from a number of drawbacks and represent only a small proportion of the total marine coatings market31. In the near future it is likely that coatings to combat marine biofouling will be based on what are considered to be 'eco-friendly' biocides (for example, by tethering to the coating or by using biocides with low toxicity and short half-lives)70. However, we have shown in this review that significant research efforts are being directed towards the discovery or improvement of novel, non-toxic, non-fouling coatings as alternatives to those currently used commercially. We have given several examples demonstrating that fouling organisms are influenced in their initial surface colonization behaviour, and the subsequent development of adhesion strength, by nano- and micro-scale-engineered coating designs. The examples chosen are not exhaustive, and there is abundant research on other ways of combating fouling organisms through non-toxic coating designs that we have not been able to discuss. For example, protein-degrading enzymes (serine proteases) have been shown to be effective in reducing settlement and adhesion strength of a range of fouling organisms, algal spores, diatoms and barnacle cyprids71, due to dissolution of adhesive72,73. However, the challenge for enzyme technology will be to achieve controlled release and stability of enzymes when incorporated into a coating74,75.
In some cases, the novel coatings we have discussed are real candidates for practical application after further development work. However, little is known about the mechanism(s) by which these engineered coatings exert their effects. We have shown several examples of correlations between specific types of morphological or chemical surface structuring and biological performance. However, correlation does not imply causality and there are real knowledge barriers to understanding how these coatings work. Advances are needed to understand the underlying biological and molecular mechanisms. For example, there is almost no information available for the common macrofouling organisms on what the critical length scale is of the structures involved in surface sensing, nor the molecular basis of their functioning (it is presumed that similar to other cell types, spores of seaweeds, barnacle larvae etc possess mechanotransducing proteins and stretch-sensitive ion channels in their surface membranes that can sense and initiate responses to surface structures, but there are no actual reports of such proteins).
We have shown in this review that there is a growing body of evidence that amphiphilic or chemically 'ambiguous' coatings present both polar and non-polar functionalities to a fouling organism and show promising performance against a wide range of fouling organisms; indeed, examples of this type of coating, such as Intersleek 900, are available commercially. However, relatively little is understood about the underlying 'design rules' or the biological basis of performance. Specifically, more understanding of coating surface properties in the immersed condition, rather than in dry or in vacuo is needed, as immersion changes the dynamics of self-assembly and surface organization. For example, in the case of coatings based on amphiphilic PEGylated and fluorinated copolymers, the hypothesis is that these are structured to present a chemically complex surface to fouling organisms. But, is the reduced adhesion observed solely due to the reduced ability of adhesive proteins to adsorb to the PEGylated domains (PEG is well known to be resistant to protein adsorption76) and what are the critical dimensions of the hydrophilic/hydrophobic surface domains? Furthermore, on immersion in the natural marine environment, the surface properties of the coatings may change further due to surface conditioning by adsorption of macromolecules and microorganisms77, which will also vary according to season and geographic location. The development of novel, nanorough, fouling-release polysiloxanes containing carbon nanotubes is intriguing, but at present there is no understanding as to the mechanistic basis of this effect; does the nanoroughness cause the cells/larvae to adhere less strongly (that is, a 'biological' basis) or is a 'physical' mechanism more likely whereby nano- and micro-air incursions, such as those postulated for SHCs36, reduce the interfacial contact between adhesive and surface, thereby reducing adhesion strength? Interdisciplinary studies combining chemistry, biology and advanced physical techniques for interfacial characterization will be crucial in advancing this field.
The control of biofouling of surfaces in the marine environment is a considerable challenge: the fouling organisms are opportunists and have evolved the capability to attach and proliferate on a range of surfaces in the natural, highly turbulent marine environment. We consider it unlikely that non-biocidal solutions based on coating designs incorporating a single attribute will solve the problem. One way forward will be to design 'multifunctional coatings', incorporating a range of attributes, for example, an appropriate topography combined with a suitable amphiphilic or zwitterionic surface chemistry and environmentally benign compounds that deter settlement or enzymes to target their bioadhesives. Bioinspired 'smart' coatings combining topography with sacrificial or renewable, low-fouling polymers to shed fouling organisms in a manner analogous to marine mammals78 may emerge as a practical concept. Fortunately, there is no shortage of innovative ideas! The challenge is to persuade funding agencies that expenditure on research and development will translate into real benefits in terms of economics and reduced environmental burdens. A recent economic analysis3 shows that a small improvement in the condition of the hulls would produce a significant saving. For example, if improved AF technology could produce just a modest reduction in overall fouling level on one class of destroyers operated by the US Navy (for example, from 'heavy slime' to 'light slime'), that alone would result in a saving of $19 M per annum!
How to cite this article: Callow, J. A. & Callow, M. E. Trends in the development of environmentally friendly fouling-resistant marine coatings. Nat. Commun. 2:244 doi: 10.1038/ncomms1251 (2011).
Schultz, M. P. Effects of coating roughness and biofouling on ship resistance and powering. Biofouling 23, 331–341 (2007). Much-quoted paper reporting the powering penalties of different scales of ship hull fouling.
Poloczanska, E. S. & Butler, A. J. Biofouling and climate change. in Biofouling (eds Durr, S. & Thomason, J.C.) (Wiley-Blackwell, 2010).
Schultz, M. P., Bendick, J. A., Holm, E. R. & Hertel, W. M. Economic impact of biofouling on a naval surface ship. Biofouling 27, 87–98 (2011)The most up-to-date and authoritative analysis of the cost of ship hull biofouling.
Jain, A. & Bhosle, N. B. Biochemical composition of the marine conditioning film: implications for bacterial adhesion. Biofouling 25, 13–19 (2009).
Chambers, L. D., Stokes, K. R., Walsh, F. C. & Wood, R. J. K. Modern approaches to marine antifouling coatings. Surf. Coat. Technol. 201, 3642–3652 (2006).
Wahl, M. Marine epibiosis 1. Fouling and antifouling—some basic aspects. Marine Ecol. Prog. Ser. 58, 175–189 (1989).
Yebra, D. M., Kiil, S. & Dam-Johansen, K. Antifouling technology-past, present and future steps towards efficient and environmentally friendly antifouling coatings. Prog. Org. Coating 53, 256–275 (2004).
Magin, C. M., Cooper, S. P. & Brennan, A. B. Non-toxic antifouling strategies. Mater. Today 13, 36–44 (2010).
Rosenhahn, A., Schilp, S., Kreuzer, H. J. & Grunze, M. The role of 'inert' surface chemistry in marine biofouling prevention. Phys. Chem. Chem. Phys. 12, 4275–4286 (2010).
Callow, M. E., Callow, J. A., Pickett-Heaps, J. D. & Wetherbee, R. Primary adhesion of Enteromorpha (Chlorophyta, Ulvales) propagules: quantitative settlement studies and video microscopy. J. Phycol. 33, 938–947 (1997).
Roberts, D., Rittschof, D., Holm, E. & Schmidt, A. R. Factors influencing initial larval settlement: temporal, spatial and surface molecular components. J. Exp. Marine Biol. Ecol. 150, 203–221 (1991).
Clare, A. S., Rittschof, D., Gerhart, D. J. & Maki, J. S. Molecular approaches to non-toxic antifouling. Invertebr. Reprod. Dev. 22, 67–76 (1992).
Rittschof, D. Research on environmentally benign antifouling coatings. In Biofouling (eds Durr S. & Thomason J.C.) (Wiley-Blackwell, 2010).
Callow, J. A. & Callow, M. E. Biofilms in Antifouling Compounds. In Progress in Molecular and Subcellular Biology: Marine Molecular Biotechnology (eds Fusetani, N. & Clare, A. S.) 141–169 (Springer, 2006).
Dobretsov, S., Dahms, H.- U. & Qian, P.- Y. Inhibition of biofouling by marine microorganisms and their metabolites. Biofouling 22, 43–54 (2006).
Huggett, M. J., Williamson, J. E., de Nys, R., Kjelleberg, S. & Steinberg, P. D. Larval settlement of the common Australian sea urchin Heliocidaris erythrogramma in response to bacteria from the surface of coralline algae. Oecologia 149, 604–619 (2006). A comprehensive study of the influence of surface biofilms on the settlement of invertebrate larvae and the first paper to show that larvae are able to respond to a bacterial biofilm in the field.
Dobretsov, S., Teplitski, M. & Paul, V. Mini-review: Quorum sensing in the marine environment and its relationship to biofouling. Biofouling 25, 413–427 (2009).
Huang, S. & Hadfield, M. G. Composition and density of bacterial biofilms determine larval settlement of the polycheate Hydroides elegans. Mar. Ecol. Prog. Ser. 260, 161–172 (2003).
Molino, P. J., Campbell, E. & Wetherbee, R. Development of the initial diatom microfouling layer on antifouling and fouling-release coatings in temperate and tropical Australia. Biofouling 25, 685–694 (2009).
Molino, P. J. et al. Development of primary bacterial microfouling layer on antifouling and fouling release coatings in temperate and tropical environments in Eastern Australia. Biofouling 25, 149–162 (2009).
Aldred, N. & Clare, A. S. Mini-review: the adhesive strategies of cyprids and development of barnacle-resistant marine coatings. Biofouling 24, 351–363 (2008).
Smith, A. M. & Callow, J. A. Biological Adhesives (Springer, 2006).
Sagert, J., Sun, C. & Waite, J. H. Chemical subtleties of mussel and polychaete holdfasts. In Biological Adhesives (eds Smith A.M. & Callow J.A.) 125–144 (Springer, 2006).
Even, M. A., Wang, J. & Chen, Z. Structural information of mussel adhesive protein Mefp-3 acquired at various polymer/Mefp-3 solution interfaces. Langmuir 24, 5795–5801 (2008). An important paper demonstrating the potential of sum frequency generation spectroscopy to study the molecular conformation of marine adhesive proteins on different polymer interfaces.
Marechal, J. P., Hellio, C., Sebire, M. & Clare, A. S. Settlement behaviour of marine invertebrate larvae measured by EthoVision 3.0. Biofouling 20, 211–217 (2004).
Aldred, N., Guozhu, L., Gao, Y., Clare, A. S. & Jiang, S. Modulation of barnacle (Balanus amphitrite Darwin) cyprid settlement behavior by sulfobetaine and carboxybetaine methacrylate polymer coatings. Biofouling 26, 673–683 (2010).
Zhang, Z., Chao, T., Chen, S. F. & Jiang, S. Y. Superlow fouling sulfobetaine and carboxybetaine polymers on glass slides. Langmuir 22, 10072–10077 (2006).
Andersson, O., Ekblad, T., Aldred, N., Clare, A. S. & Liedberg, B. Novel application of imaging surface plasmon resonance for in situ studies of the surface exploration of marine organisms. Biointerphases 4, 65–68 (2009). Novel application of iSPR to study the deposition of adhesive 'footprints' of barnacle larvae as they explore surfaces with different physico-chemical properties, in situ and in real time.
Kavanagh, C. J., Quinn, R. D. & Swain, G. W. Observations of barnacle detachment from silicones using high-speed video. J. Adhes. 81, 843–868 (2005).
Tribou, M. & Swain, G. The use of proactive in-water grooming to improve the performance of ship hull antifouling coatings. Biofouling 26, 47–56 (2010).
Finnie, A. & Williams, D. N. Paint and coatings technology for the control of marine fouling. In: Biofouling (eds Durr S. & Thomason J.C.) (Wiley-Blackwell, 2010).
Brady, R. F. & Singer, I. L. Mechanical factors favoring release from fouling release coatings. Biofouling 15, 73–81 (2000). Seminal paper on the fracture mechanics involved in the release of 'hard-fouling' from elastomeric coatings.
Anderson, C., Atlar, M., Callow, M., Candries, M. & Townsin, R. L. The development of foul-release coatings for seagoing vessels. J. Mar. Des. Oper. 84, 11–23 (2003).
Bers, A. V., D'Souza, F., Klijnstra, J. W., Willemsen, P. R. & Wahl, M. Chemical defence in mussels: antifouling effect of crude extracts of the periostracum of the blue mussel Mytilus edulis. Biofouling 22, 251–259 (2006).
Ralston, E. & Swain, G. Bioinspiration- the solution for biofouling control? Bioinspir. Biomim. 4, 1–9 (2009).
Scardino, A. J., Zhang, H., Cookson, D. J., Lamb, R. N. & de Nys, R. The role of nano-roughness in antifouling. Biofouling 25, 749–756 (2009).
Genzer, J. & Marmur, A. Biological and synthetic self-cleaning surfaces. MRS Bull. 33, 742–746 (2008).
Scardino, A. J. & de Nys, R. Biomimetic models and bioinspired surfaces for fouling control. Biofouling 27, 73–86 (2011).The most recent and extensive review of natural antifouling defences and their potential for biomimetic antifouling solutions.
Carman, M. L. et al. Engineered antifouling microtopographies—correlating wettability with cell attachment. Biofouling 22, 11–21 (2006).
Scardino, A., Guenther, J. & de Nys, R. Attachment point theory revisited: the fouling response to a microtextured matrix. Biofouling 24, 45–53 (2008).
Schumacher, J. F. et al. Engineered antifouling microtopographies-effect of feature size, geometry and roughness on settlement of zoospores of the green alga Ulva. Biofouling 23, 55–62 (2007). This paper describes the outcome of a systematic approach to understand the role of surface roughness in the attachment of a marine species resulting in a novel quantitative model (the Engineered Roughness Index).
Long, C. J. et al. A model that predicts the attachment behavior of Ulva linza zoospores on surface topography. Biofouling 26, 411–419 (2010).
Schumacher, J. F. et al. Engineered nanoforce gradients for inhibition of settlement (attachment) of swimming algal spores. Langmuir 24, 4931–4937 (2007).
Schumacher, J. F. et al. Species-specific engineered antifouling topographies: correlations between the settlement of algal zoospores and barnacle cyprids. Biofouling 23, 307–317 (2007).
Lin, F.- Y., Chen, W.- Y. & Hearn, M. T. W. Thermodynamic analysis of the interaction between proteins and solid surfaces: application to liquid chromatography. J. Mol. Recognit. 15, 55–93 (2002).
Krishnan, S. et al. Anti-biofouling properties of comb-like block copolymer with amphiphilic side-chains. Langmuir. 22, 5075–5086 (2006).
Lau, K. H. A., Bang, J., Hawker, C. J., Kim, D. H. & Knoll, W. Modulation of protein-surface interactions on nanopatterned polymer films. Biomacromolecules 10, 1061–1066 (2009). Why do some heterogeneous, nanopatterned films, such as those spontaneously generated from block copolymers, show reduced adsorption of proteins? This paper systematically explores the influence of feature size on protein adsorption and relates performance to the density of surface interfaces.
Gudipati, C. S., Greenleaf, C. M., Johnson, J. A., Pryoncpan, P. & Wooley, K. L. Hyperbranched fluoropolymer and linear poly(ethyleneglycol) based amphiphilic cross-linked networks as efficient antifouling coatings: An insight into the surface compositions, topographies and morphologies. J. Polym. Sci. A Polym. Chem. 42, 6193–6208 (2004).
Gudipati, C. S., Finlay, J. A., Callow, M. E., Callow, J. A. & Wooley, K. L. The anti-fouling and fouling-release performance of unique hyperbranched fluoropolymer (HBFP)-poly(ethylene glycol) (PEG) composite coatings evaluated by protein adsorption and the settlement of zoospores of the green fouling alga Ulva (syn. Enteromorpha). Langmuir 21, 3044–3053 (2005).
Krishnan, S. et al. Comparison of the fouling release properties of hydrophobic fluorinated and hydrophilic PEGylated block copolymer surfaces: attachment strength of the diatom Navicula and the green alga Ulva. Biomacromolecules 7, 1449–1462 (2006).
Martinelli, E. et al. Nanostructured films of amphiphilic fluorinated block copolymers for fouling release application. Langmuir 24, 13138–13147 (2008).
Martinelli, E. et al. Surface engineering of styrene/PEGylated-fluoroalkyl styrene block copolymer thin films. J. Polym. Sci. A Polym. Chem. 47, 267–284 (2009).
Weinman, C. J. et al. ABC triblock surface active block copolymer with grafted ethoxylated fluoroalkyl amphiphilic side chains for marine antifouling/fouling-release applications. Langmuir 25, 12266–12274 (2009).
Kristalyn, C. B. et al. Surface structures of an amphiphilic tri-block copolymer in air and in water probed using sum frequency generation vibrational spectroscopy. Langmuir 26, 11337–11343 (2010).
Majumdar, P. & Webster, D. C. Preparation of siloxane-urethane coatings having spontaneously formed stable biphasic microtopograpical surfaces. Macromolecules 38, 5857–5859 (2005).
Majumdar, P. & Webster, D. C. Influence of solvent composition and degree of reaction on the formation of surface microtopography in a thermoset siloxane–urethane system. Polymer 47, 4172–4181 (2006).
Majumdar, P., Stafslien, S., Daniels, J. & Webster, D. C. High throughput combinatorial characterization of thermosetting siloxane–urethane coatings having spontaneously formed microtopographical surfaces. J. Coat. Technol. Res. 4, 131–138 (2007).
Sommer, S. et al. A preliminary study on the properties and fouling-release performance of siloxane-polyurethane coatings prepared from poly(dimethylsiloxane) (PDMS) macromers. Biofouling 26, 961–972 (2010).
Majumdar, P. et al. Combinatorial materials research applied to the development of new surface coatings IX: an investigation of novel antifouling/fouling-release coatings containing quaternary ammonium salt groups. Biofouling 24, 185–200 (2008).
Jiang, S. & Cao, Z. Ultralow-fouling, functionalizable and hydrolysable zwitterionic materials and their derivities for biological applications. Adv. Mater. 22, 920–932 (2010).
Zhang, Z. et al. Polysulfobetaine-grafted surfaces as environmentally benign ultra-low fouling marine coatings. Langmuir 25, 13516–13521 (2009). First paper to demonstrate the settlement-inhibiting effect of tethered zwitterionic coatings for a marine species.
Bennett, S. M. et al. The role of surface energy and water wettability in aminoalkyl/fluorocarbon/hydrocarbon-modified xerogel surfaces in the control of marine biofouling. Biofouling 26, 235–246 (2010).
Tang, Y. et al. Hybrid xerogel films as novel coatings for antifouling and fouling release. Biofouling 21, 59–71 (2005).
McMaster, D. M. et al. Antifouling character of 'active' hybrid xerogel coatings with sequestered catalysts for the activation of hydrogen peroxide. Biofouling 25, 21–33 (2009).
Finlay, J. A. et al. Barnacle settlement and the adhesion of protein and diatom microfouling to xerogel films with varying surface energy and water wettability. Biofouling 26, 657–666 (2010).
Beigbeder, A. et al. Preparation and characterisation of silicone-based coatings filled with carbon nanotubes and natural sepiolite, and their application as marine fouling-release coatings. Biofouling 24, 291–302 (2008).
Begbeider, A. et al. CH-p interactions as the driving force for silicone-based nanocomposites with exceptional properties. Adv. Mater. 20, 1003–1007 (2008).
Beigbeder, A. et al. On the effect of carbon nanotubes on the wettability and surface morphology of hydrosilylation-curing silicone coatings. J. Nanostruct. Polym. Nanocomposites 5, 37–43 (2009).
Marmur, A. Adhesion and wetting in an aqueous environment: theoretical assessment of sensitivity to the solid surface energy. Langmuir 20, 1317–1320 (2004).
Thomas, K. V. & Brooks, S. The environmental fate and effects of antifouling paint biocides. Biofouling 26, 73–88 (2010).
Pettitt, M., Henry, S., Callow, M., Callow, J. & Clare, A. Activity of commercial enzymes on settlement and adhesion of cypris larvae of the barnacle Balanus amphitrite, spores of the green alga Ulva linza, and the diatom Navicula perminuta. Biofouling 20, 299–311 (2004).
Phang, I. Y. et al. Atomic force microscopy of the morphology and mechanical behaviour of barnacle cyprid footprint proteins at the nanoscale. J. Roy. Soc. Interface 24, 97–107 (2008). First application of AFM to study the mechanical properties of the temporary footprint adhesive deposited by barnacle larvae as they explore surfaces.
Aldred, N., Phang, I. Y., Conlan, S. L., Clare, A. S. & Vansco, G. J. The effects of a serine protease, Alcalase® on the adhesives of barnacle cyprids (Balanus amphitrite). Biofouling 24, 97–107 (2008).
Tasso, M. et al. Antifouling potential of Subtilisin A immobilized onto maleic anhydride copolymer thin films. Biofouling 25, 505–516 (2009).
Tasso, M., Cordeiro, A. L., Salchert, K. & Werner, C. Covalent immobilization of Subtilisin A onto Thin Films of Maleic Anhydride Copolymers. Macromol. Biosci. 9, 922–929 (2009).
Jeon, S. I., Lee, J. H., Andrade, J. D. & de Gennes, P. G. Protein-surface interactions in the presence of polythylene oxide: I. Simplified theory. J. Colloid Interface Sci. 142, 149–158 (1991).
Huggett, M. J., Nedved, B. T. & Hadfield, M. G. Effects of initial surface wettability on biofilm formation and subsequent settlement of Hydroides elegans. Biofouling 25, 387–399 (2009).
Ganguli, R., Mehrotra, V. & Dunn, B. Bioinspired living skins for fouling mitigation. Smart Mater. Struct. 18, 1–8 (2009).
The authors acknowledge support from the AMBIO project (NMP-CT-2005-011827) funded by the European Commission's 6th Framework Programme, and support from the US Office of Naval Research (N00014-08-1-0010). Dr Nick Aldred and Professor Tony Clare (University of Newcastle) are thanked for comments on Figures 3 and 4.
The authors declare no competing financial interests.
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Callow, J., Callow, M. Trends in the development of environmentally friendly fouling-resistant marine coatings. Nat Commun 2, 244 (2011). https://doi.org/10.1038/ncomms1251
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