Figure 1: Schematic overview of RACE-seq. | Nature Communications

Figure 1: Schematic overview of RACE-seq.

From: Extension of human lncRNA transcripts by RACE coupled with long-read high-throughput sequencing (RACE-Seq)

Figure 1

Standard 5′ and 3′ RACE primers are designed to target exons of a gene and produce primary RACE products, which undergo a second round of RACE reactions using nested 5′ or 3′ RACE primers. Both standard and nested 5′ and 3′ RACE products are subjected to long-read sequencing, followed by mapping to the reference genome.

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