Figure 8 : CRY1 alleviates gluconeogenesis in diabetic mouse models.

From: SREBP1c-CRY1 signalling represses hepatic glucose production by promoting FOXO1 degradation during refeeding

Figure 8

(ac) Eight-week-old male mice were injected STZ (150 mg kg−1) and sacrificed in ZT 3 after 1 week later. Blood glucose levels (a) were measured in ad libitum at ZT 3. After all of the mice were sacrificed at ZT 3, hepatic protein levels (b) were analysed by western blotting. The mRNA levels (c) were determined by qRT-PCR analyses and normalized to the TBP mRNA level. Data represent mean ±s.d., N=3 for each group. *P<0.05, **P<0.01, ***P<0.001 (Student’s t-test). (df) STZ-injected mice were infected with adenovirus encoding MOCK or CRY1 (adenoviral dose of 2 × 109 viral particles per mouse). The blood glucose levels (d) were measured in ad libitum at ZT 3. After all of the mice were sacrificed at ZT 3, the mRNA levels (e) were determined by qRT-PCR analyses and normalized to the TBP mRNA level. Hepatic protein levels (f) were analysed by western blotting. Data represent mean ±s.d., N=4 for each group. *P<0.05, **P<0.01 (Student’s t-test). (g,h) Ten-week-old male db/+ and db/db mice were sacrificed in ad libitum at ZT 3. The relative mRNA levels of various hepatic genes (g) were determined by qRT-PCR analyses and normalized to the TBP mRNA level. Data represent mean ±s.d., N=4 for each group. *P<0.05 (Student’s t-test). Protein levels (h) were determined with western blotting. (il) Ten-week-old male db/db mice were infected through the tail vein with adenovirus encoding MOCK or CRY1 (adenoviral dose of 2 × 1010 viral particles per mouse) and subjected to the pyruvate tolerance test (i). All mice were fasted at ZT 10 and performed PTT at ZT 3. Results were converted to AUC. The blood glucose levels (j) were measured in ad libitum at ZT 3. After all of the mice were sacrificed at ZT 3, hepatic protein levels (k) were analysed by western blotting. The mRNA levels (l) were determined by qRT-PCR analyses and normalized to the TBP mRNA level. Data represent mean ±s.d., N=5 for each group. *P<0.05, **P<0.01, ***P<0.001 (Student’s t-test). See Supplementary Fig. 13 for original full immunoblot.