Figure 1: Summary of experimental design and analysis. | Nature Communications

Figure 1: Summary of experimental design and analysis.

From: Host genotype and age shape the leaf and root microbiomes of a wild perennial plant

Figure 1

(a) Map of the study region in central Idaho, USA (map data from the R package ‘maps’70). The five genotypes used in this experiment were collected from the five B. stricta populations shown. We collected seeds from 8–10 individual B. stricta plants from each population, for a total of 48 accessions or genetic ‘lines’. For our analyses these lines were grouped back into five ‘genotypes’ corresponding to the populations from which their ancestors were collected. The populations marked with triangles correspond to the ‘sites’ of the three common gardens where the experiment took place. Scale bar, 50 km. (b) Schematic representation of common garden layout. Each garden contained six replicated, randomized blocks per planting cohort (2008 and 2009). Each block contained one replicate of each ‘line’, for a total of 8–10 replicates per ‘genotype’. In both 2011 and 2012, one individual of each genotype was haphazardly chosen for destructive sampling in each block. (c) A temporally staggered planting/harvesting design disentangled the effects of plant age and year of observation. (d) Abbreviations and colour codes are shown for the five genotypes and three sites featured in this study. (e) The relative abundances of major phyla are shown for each leaf or root sample.

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