Figure 3 : LCM-seq revealed the unique identities of cervical and lumbar spinal MNs.

From: Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling

Figure 3

(a) PCA for cSC (n=4 mice) and lSC (n=5 mice) MNs (120 neurons/sample) based on the top 500 variable genes in expression showed that the two groups separated along the 1st component. (b) Genes (899) were differentially expressed between cSC MNs and lSC MNs (adjusted P<0.05, Wald test). Heatmap shows the Z-score of each gene, which was calculated based on log10-transformed RPKM values. (c) cSC MNs and lSC MNs showed unique Hox gene expression profiles. (d) The top 20 differentially expressed genes between cSC and lSC neurons included eight Hox genes, and for example, Npy, Fstl1 and Sncb (sorted by fold change and adjusted P, shown as mean±s.e.m.). (e) The top 20 differentially expressed transcription factors (TFs) between cSC and lSC neurons included twelve Hox genes and for example, Nr2f2 and Zfhx4 (sorted by fold change and adjusted P, shown as mean±s.e.m.).