Figure 1 : Schematics of Smart-seq2 coupled with LCM (LCM-seq) for analysis of single neurons.

From: Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling

Figure 1

Spinal cords isolated from mouse pups were sectioned at 12 μm thickness at cervical and lumbar levels. MNs were visualized using Histogene staining, 120, 50, 30, 10, 5, 2 and 1 cell captured by a Leica LMD7000 system and collected in PCR tubes. Cells were lysed, cDNA synthesized, amplified and analysed using a Bioanalyzer and sequencing libraries subsequently prepared. The cDNA profile is exemplified by a Bioanalyzer profile of 50 captured MNs. bp, basepairs; FU, fluorescent units.