Table 1 SIV Env reactive mAbs isolated from chronically SIV-infected AGMs.

From: Envelope-specific B-cell populations in African green monkeys chronically infected with simian immunodeficiency virus

Ab ID V H HCDR 3 (AA) H Mut (%) Isotype V L LCDR 3 (AA) L Mut (%) Sample Type EC50 (μg ml−1) Neutralization (IC50, μg ml−1)
          gp120 V1V2 V3 gp140 sp400 SIVmacTCLA SIVsab92018
DH543 5–13 12 6.6 G Vλ1–73 11 7.1 Milk >100 >100 >100 0 .009 0.053 >50 >50
DH544 3–22 11 8.5 G Vλ1–57 11 5.2 Milk >100 >100 >100 0.005 0.087 >50 >50
DH545 4–152 9 15.6 G Vκ2–92 9 7.5 Milk >100 >100 >100 0.003 0.07 >50 >50
DH546 4–152 19 11.8 G Vλ1–73 11 9.4 Milk 0.37 >100 >100 0.021 >100 >50 >50
DH547 1–93 19 11.8 G Vλ1–73 12 4.1 Milk 0.054 >100 0.497 0.005 >100 >50 >50
DH548 4–76 15 12.4 G Vλ3–36 11 5.0 Blood 0.004 >100 >100 <0.001 >100 >50 >50
DH549 3–67 13 5.9 G Vκ1–NL2 9 6.1 Blood 0.001 >100 >100 0.002 >100 <0.02 >50
DH550 3–67 12 12.5 G Vκ3–53 9 4.2 Blood 0.002 >100 >100 0.002 >100 >50 >50
DH551 4–88 19 13.2 G Vκ1–39 9 3.8 Blood 0.004 >100 2.229 0.003 >100 <0.02 >50
DH552 4–88 17 7.3 G Vλ1–73 11 2.6 Blood 5.899 >100 >100 0.397 >100 >50 >50
  1. AGM, African green monkey; HCDR, heavy chain complementarity-determining region; LCDR, light chain complementarity-determining region; mAb, monoclonal antibody; SIV, simian immunodeficiency virus; TCLA, T-cell line-adapted; VH, heavy chain variable; VL, light chain variable.
  2. High binding strength (EC50) and potent neutralizing activity (IC50) are denoted in bold.