Figure 3 : Gliosis but no change in GABAergic neuron density are visible in FCDs.

From: Convulsive seizures from experimental focal cortical dysplasia occur independently of cell misplacement

Figure 3

(a,b) GFAP immunostaining (green) in coronal sections containing electroporated neurons (red) under control (a) or RhebCA condition (b). The dotted line and white arrows point to a bulging of the ACC containing RhebCA-expressing cytomegalic neurons. Scale bar, 150 μm. (c) Bar graphs of fluorescence (Fu) intensity of GFAP immunoreactivity in both the ipsilateral (ipsi) and contralateral (contra) cortex under conditions shown in (a,b). P<0.01 (two-way analysis of variance (ANOVA)); *P<0.05, **P<0.01 (Sidak's multiple comparisons post-test). (d) T1-weighted MR images of control (d) and RhebCA (e) brains showing measuring bars for cortical thickness. (e) Quantification of cortical thickness shown in d,e with N=6 per condition. P<0.001 (two-way ANOVA); ***P<0.001 (Sidak’s multiple comparisons post-test). (f) Serial tomograms from diffusion tensor imaging (DTI) with statistically significant (P<0.01) differences in fractional anisotropy (FA; RhebCA minus control, expressed in heat maps with scale at the bottom) between control (N=4) and RhebCA (N=4) transfected brains, overlaid on top of anatomical scans of a control brain. (g) Top, GABA immunostaining in coronal sections from mice electroporated with control or RhebCA plasmid. Scale bar, 300 μm. Bottom, bar graph of the density of GABAergic cells in control and RhebCA-expressing sections. Error bars, s.e.m.