Figure 2 : T1D SC-β cells function rapidly and persist several months in vivo after transplantation.

From: Generation of stem cell-derived β-cells from patients with type 1 diabetes

Figure 2

(a) Schematic summarizing in vivo transplantation experiments. ms, endogenous mouse β cells. T1D, T1D SC-β cells. (b) Average ELISA measurements of serum human insulin before (0 min) and 30 min after a glucose injection for mice that received T1D and ND SC-β cells 2 week prior. n=32 and 48 for T1D and ND SC-β cells, respectively. (c) Representative immunostaining of grafts 2 weeks after transplantation stained with glucagon (GCG; red), C-peptide (green) and 4,6-diamidino-2-phenylindole (DAPI) (blue). (d) Average ELISA measurements of serum human insulin before (0 min) and 30 min after a glucose injection for mice that received T1D and ND SC-β cells 12–16 weeks prior. n=15 and 18 for T1D and ND SC-β cells, respectively. (e) Representative immunostaining of grafts 12–16 weeks after transplantation. (f) Fasting blood glucose measurements for a subset of mice that received an alloxan injection to destroy endogenouse mouse β cells. Mice that were transplanted with ND SC-β cells (closed circle; n=4) and with T1D SC-β cells (open triangle; n=4) are shown. (g) Average ELISA measurements of serum human insulin before (0 min) and 30 min after a glucose injection for mice that were injected with alloxan 80 days prior. n=4 and 4 for T1D and ND SC-β cells, respectively. (h) Temporal blood glucose measurements after a glucose injection 29 days after alloxan treatment. Shown are mice that had not received a transplantation (open diamond; n=2), were transplanted with ND SC-β cells (closed circle; n=4), were transplanted with T1D SC-β cells (open triangle; n=4), or did not receive alloxan treatment and did not receive a transplantation (closed square; n=5) are shown. (i) Temporal blood glucose measurements after a glucose injection 80 days after alloxan treatment. Mice that were transplanted with ND SC-β cells (closed circle; n=4), were transplanted with T1D SC-β cells (open triangle; n=4), or did not receive alloxan treatment and did not receive a transplantation (closed square; n=4) are shown. Mice were transplanted with cells 10–17 days in Stage 6. Scale bar, 100 μm. *P<0.05 and **P<0.01 (two-sided paired t-test). Data shown as mean±s.e.m.