Quantitative RT–PCR-based expression profiling of the following genes in glucose-sensitive insulin-secreting beta-like cells differentiated by the lineage-control network: (a) the key pancreatic beta-cell-specific transcription factors Glis3, MafA, MafB, Mnx1, NeuroD, Pax4, Pdx1 and Nkx6.1, (b) the glucose- and insulin-processing factors Gck, Glut2, G6pc2, Pcsk1, Pcsk2, Slc30a8, Snap25, Stx1A, Stxbp1, Syt4, (c) the channels essential for the secretion of insulin such as Abcc8, Cacna1D, Kcnk1/3 and Kcnj11 and (d) the human islet peptide hormones Chgb, Ghrelin, Glucagon, Iapp, Insulin and Somatostatin, and (e) immature as well as mature human pancreatic beta-cell markers Acox2, Ck19, Dpp4, FoxA1, Fzd2, Gcgr, Irx2, Mmp2, Onecut2, Sftpd and Ucn3. The transcript levels were profiled at day 11 relative to hIPSCs and normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Data are the means±s.d. of triplicate experiments (n=9). Abcc8, ATP-binding cassette transporter sub-family C member 8; Acox2, Acyl-CoA oxidase 2; Cacna1D, voltage-dependent, L-type alpha 1D subunit; Chgb, chromogranin B; Ck19, cytokeratin-19; Dpp4, dipeptidyl-peptidase 4; FoxA1, forkhead box protein A1; Fzd2, frizzled 2; Gcgr, glucagon receptor; Gck, glucokinase; Glis3, glis family zinc finger 3; Glut2, glucose transporter 2; G6pc2, glucose-6-phosphatase 2; Iapp, islet amyloid polypeptide; Irx2, iroquois homeobox 2; Kcnk1/3, potassium channel, subfamily K, member 1/3; Kcnj11, potassium inwardly-rectifying channel, subfamily J, member 11; MafA/B, V-maf musculoaponeurotic fibrosarcoma oncogene homologue A/B; Mmp2, matrix metalloproteinase 2; Mnx1, motor neuron and pancreas homeobox 1; NeuroD1, neurogenic differentiation factor 1; Nkx6.1, NK6 homeobox 1; Onecut2, onecut homeobox 2; Pax4, paired box gene 4; Pcsk1/2, proprotein convertase 1/2; Sftpd, surfactant protein D; Pdx1, pancreatic and duodenal homeobox 1; Slc30a8, solute carrier family 30, member 8; Snap25, synaptosomal-associated protein; Stx1A (Syntaxin-1A), Stxbp1, syntaxin binding protein 1; Syt4 synaptotagmin-4; Ucn3, urocortin 3.