Figure 5 : Analysis of global transcription reveals a regeneration-specific transcriptional response associated with ECM-related genes.

From: Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals

Figure 5

(a) Ear-hole area following 4-mm ear-hole punch assay in M. musculus (blue) and A. cahirinus (red). Data represent box and whisker plots from male and female individuals re-plotted from Fig. 1a. Adjacent images of representative ears at D85 for M. musculus (top) and A. cahirinus (bottom). Blue and red circles indicate initial wound. Regenerated hair follicles are present in A. cahirinus. (b) Heatmap showing expression relative to D0 for global transcription from M. musculus (M) and A. cahirinus (A) at D5, D10, D15 and D20. Tree to the right of heatmap represents the ontogeny of gene expression and each colour is used to separate the genes into 20 unique clusters via hierarchical clustering based on expression pattern (C3/C4=up in M, no change in A; C6/C10=no change in M, up in A; C12=down in M, up in A; C15=down in M, up in A; C17/C18/C19/C20=no change in M, down in A). (c) Heatmap for 204 out of 277 genes from the core murine matrisome (glycoproteins, collagens and proteoglycans). Data represent log2(mean expression) calculated from EBseq averaged over n=3 biological replicates at each time point per species (b,c). (d) Quantitative PCR results for select genes from matrisome. Data represent mean and s.e.m. for n=3 biological replicates at each time point per species.