Figure 6 : Specific and unspecific labelling.

From: Targeting neurotransmitter receptors with nanoparticles in vivo allows single-molecule tracking in acute brain slices

Figure 6

(a) Some QD functionalized with anti-GFP were found to diffuse over large dendritic areas, inside and outside spines of neurons transfected with D1-CFP (scale bar, 2 μm). QD functionalized with anti‐Flag antibodies did not show diffusion patterns along D1‐CFP transfected neurons, but mainly appeared immobile when co-localizing with transfected neurons. The same immobile pattern was observed with QD functionalized with anti-GFP over neurons that were not transfected with D1-CFP. (b) A quantification of these different behaviours can be seen in the cumulative distribution of diffusion coefficients.