Figure 6 : Transcriptome analysis reveals preferentially regulated and commonly regulated genes by MML-1/MXL-2 and HLH-30.

From: Mondo complexes regulate TFEB via TOR inhibition to promote longevity in response to gonadal signals

Figure 6

(a) Venn diagrams depict the numbers of DEGs identified through RNA-seq analysis from glp-1mml-1 versus glp-1, glp-1mxl-2 versus glp-1 and glp-1;hlh-30 versus glp-1. (b) GO enrichment analysis using DAVID. The enrichment (upper x axis), fold change (colour coding) and number of genes are shown (next to each bar). See Supplementary Data 5 for details. (c) qRT–PCR analysis confirms hlh-30 preferentially regulated genes (lgg-1), hlh-30/mml-1/mxl-2 commonly regulated genes (tts-1) and mml-1/mxl-2 preferentially regulated genes (lgg-2, mdl-1, swt-1 and fat-5). The bar represents mean±s.e.m. from three biological replicates relative to N2. P-value (* P<0.05, **P<0.01 and ***P<0.001) was determined by one-way analysis of variance (ANOVA) with Tukey’s test relative to glp-1.