Figure 4 : MML-1/MXL-2 promote autophagy.

From: Mondo complexes regulate TFEB via TOR inhibition to promote longevity in response to gonadal signals

Figure 4

(a) Representative fluorescent images of LGG-1::GFP foci in indicated genotypes at L4 stage. LGG-1::GFP puncta are reduced in glp-1mml-1 and glp-1mxl-2 compared with glp-1. (b) Quantification of LGG-1::GFP puncta in seam cells. The bar represents mean±s.e.m. from 3 biological replicates (>20 worms each). P-value (*P<0.05, **P<0.01) relative to N2 or glp-1 was determined by t-test. (c) Representative fluorescent images of SQST-1/p62::GFP transgenic worm of indicated genotypes at L4 stage. SQST-1::GFP aggregates are elevated in double mutants compared with glp-1. (d) Quantification of SQST-1/p62::GFP foci in the pharyngeal region. The bar represents mean±s.e.m. from 3 experiments (>20 worms each). P-value (**P<0.01) relative to N2 or glp-1 was determined by t-test. (e) Representative western blotting showing SQST-1::GFP and loading control, b-actin bands in indicated genotypes (left). SQST-1::GFP is elevated in glp-1mml-1 and glp-1mxl-2. Quantification of SQST-1::GFP bands is shown (right). Bar graphs show mean±s.e.m. relative to glp-1 from three independent experiments. Band intensity of SQST-1::GFP is normalized to loading control, b-actin. P-value (*P<0.05) is determined by t-test relative to glp-1. Scale bars, 20 μm.