Figure 3 : Mutual regulation of MML-1 and HLH-30.

From: Mondo complexes regulate TFEB via TOR inhibition to promote longevity in response to gonadal signals

Figure 3

(a) Representative fluorescent images showing HLH-30::GFP expression in day 1 young adult worms of indicated genotypes. Worms were raised at non-permissive temperatures (25 °C) for glp-1. Nuclear localization of HLH-30::GFP in glp-1 is abolished in glp-1mml-1 and glp-1mxl-2 animals. (b) Percentage of worms with HLH-30::GFP in the nuclei of intestinal cells at day 1 young adult stage (mean±s.e.m. from 3 biological replicates, >20 worms each, **P<0.01, t-test relative to glp-1). (c) Representative fluorescent images of MML-1::GFP transgenic worms in N2 and glp-1 background fed with control L4440::luc RNAi or hlh-30 RNAi. Upregulation of nuclear MML-1::GFP in glp-1 is abolished on hlh-30 knockdown. (d) Nuclear MML-1::GFP intensity relative to N2 fed with control RNAi (mean±s.e.m. from 3 biological replicates, >20 worms each, *P<0.05, t-test relative to glp-1 fed with control RNAi). Scale bars, 50 μm.