Figure 4 : Structural basis of RNA recognition by T-STAR and Sam68.

From: Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68

Figure 4

(a) Consensus binding site for T-STAR derived from alignment of full-length CLIP tags and maximum distance between two (A/U)AA sequences in each tag plotted against the normalized number of tags for T-STAR CLIP tags (blue), and random genomic region (green) as control. (b) Close-up view of the specific recognition of A2 and A3. (c) Close-up view of the specific recognition of A1 or U1. (d) NMR chemical shift perturbation upon addition of AAAUAA as a function of T-STAR KH amino acid sequence. I97 whose peak disappears upon complex formation is shown in red. (e) NMR chemical shift perturbation upon addition of AUUAAA as a function of Sam68 STAR amino acid sequence. Peaks that disappear upon complex formation are shown in red.