Figure 9: Transgenic potato expressing Pi04314 enhances P. infestans leaf colonization and attenuates induction of JA- and SA-responsive genes. | Nature Communications

Figure 9: Transgenic potato expressing Pi04314 enhances P. infestans leaf colonization and attenuates induction of JA- and SA-responsive genes.

From: A Phytophthora infestans RXLR effector targets plant PP1c isoforms that promote late blight disease

Figure 9

(a) Representative leaf images, exposed to UV, showing increased lesion sizes on Pi04314-expressing transgenic lines E-6 and OE-8, compared with the untransformed control, cv E3. (b) Mean lesion diameter is significantly increased in OE-6 and OE-8 lines compared with the control, cv E3. Letters denote statistical significance (ANOVA, P<0.001) from three biological replicates, each containing inoculation of three leaves from each of the six plants. (c) Relative expression of flg22 marker genes StWRKY8 and StACRE31 30 min after treatment with flg22 in E3, OE-6 and OE-8 lines, compared with untreated lines (which was given a value of 1). (d) Relative expression of JA-responsive genes StJAZ1-like (StJAZ1L) and StMYC2L 1 h after treatment with meJA in E3, OE-6 and OE-8 lines, compared with untreated lines (which was given a value of 1). (e) Relative expression of SA-responsive genes StWRKY40-like (StWRKY40L) and StWRKY16L 1 h after treatment with SA in E3, OE-6 and OE-8 lines, compared with untreated lines (which was given a value of 1). Results in B-E are the mean of three independent biological replicates. Error bars show s.e. and * denotes significantly reduced induction (ANOVA, P<0.001) of responsive genes in transgenic lines compared with the E3 control.

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