(a) Flight initiation assay37. (b) Results from the flight initiation assay displayed as box plots. The box marks the 25th and 75th percentile and the median (middle line). The whiskers mark the 10th and 90th percentile. Triple asterisk, P<0.001; unpaired t-test compared with wild type; n=27 (Df(3L)BSC441/piggyBac), 41 (Df(3L)ED217/piggyBac), 51 (pBac/pBac), 46 pBac/TM3), 34 (excision), 84 (minos/minos), 118 (wild type). (c) Force step (lower trace)-evoked CAP responses (middle traces) are absent in the antennal nerves of f02655 DCX-EMAP mutants (blue), whereas robust CAP responses can be recorded from wild-type (grey) and f02655 excision controls (green). Upper panel: on step actuation, the movement of the antennal receivers of controls, but not mutants, displays the characteristic overshoot that associates with the opening of transduction channels. (d) Unstimulated fluctuations of the antennal receivers of f02655 DCX-EMAP mutants and controls. Colour code as in (c).