(a) The exon–intron structure of CG42247 showing the piggyBac (f02655) insertion into the intron between exons 4 and 5. (b) The domain structure of the DCX-EMAP protein. Two (one for the truncated form) N-terminal doublecortin domains (DCX) are followed by a HELP domain. The C terminus of the 819 aa protein consists of 10 WD40 domains, shown in grey. (c) The EMAP family tree (S. purpuratus, C. elegans, D. melanogaster, D. rerio) and the five mammalian (mouse EML 1-5) EMAP homologues are shown (similarity matrix, blosum; parameters: neighbour joining; tie breaking, systematic). (d–f) U2OS cells expressing α-tubulin-mCherry (d) transfected with DCX-EMAP-EGFP (e), and overlay (f) showing co-localization between microtubules and DCX-EMAP and large microtubule bundles not observed in non-transfected cells (scale bar, 20 μm).