Biochar built soil carbon over a decade by stabilizing rhizodeposits

Abstract

Biochar can increase the stable C content of soil. However, studies on the longer-term role of plant–soil–biochar interactions and the consequent changes to native soil organic carbon (SOC) are lacking. Periodic 13CO2 pulse labelling of ryegrass was used to monitor belowground C allocation, SOC priming, and stabilization of root-derived C for a 15-month period—commencing 8.2 years after biochar (Eucalyptus saligna, 550 °C) was amended into a subtropical ferralsol. We found that field-aged biochar enhanced the belowground recovery of new root-derived C (13C) by 20%, and facilitated negative rhizosphere priming (it slowed SOC mineralization by 5.5%, that is, 46 g CO2-C m−2 yr−1). Retention of root-derived 13C in the stable organo-mineral fraction (<53 μm) was also increased (6%, P < 0.05). Through synchrotron-based spectroscopic analysis of bulk soil, field-aged biochar and microaggregates (<250 μm), we demonstrate that biochar accelerates the formation of microaggregates via organo-mineral interactions, resulting in the stabilization and accumulation of SOC in a rhodic ferralsol.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Figure 1: Proposed mechanisms for positive rhizosphere priming of soil organic carbon (SOC) counteracted by biochar-induced negative priming and stabilization of rhizodeposits (new C) in a ferralsol after 9.5 years.
Figure 2: Change in rhizosphere priming of soil organic carbon (SOC) between 8.2 and 9.5 years post biochar addition.
Figure 3: Change in total soil C stocks and root biomass C in the unamended control and field-aged biochar-amended plots.
Figure 4: Spectroscopic analysis of bulk planted soils in the unamended control and field-aged biochar-amended plots.

References

  1. 1

    Lal, R. Managing soils and ecosystems for mitigating anthropogenic carbon emissions and advancing global food security. BioScience 60, 708–721 (2010).

    Article  Google Scholar 

  2. 2

    Antle, J. M., Capalbo, S. M., Mooney, S., Elliott, E. T. & Paustian, K. H. Economic analysis of agricultural soil carbon sequestration: an integrated assessment approach. J. Agric. Res. Econ. 26, 344–367 (2001).

    Google Scholar 

  3. 3

    Ogle, S. M., Breidt, F. J. & Paustian, K. Agricultural management impacts on soil organic carbon storage under moist and dry climatic conditions of temperate and tropical regions. Biogeochemistry 72, 87–121 (2005).

    Article  Google Scholar 

  4. 4

    Stocking, M. A. Tropical soils and food security: the next 50 years. Science 302, 1356–1359 (2003).

    CAS  Article  Google Scholar 

  5. 5

    Smith, P. Soil carbon sequestration and biochar as negative emission technologies. Glob. Change Biol. 22, 1315–1324 (2016).

    Article  Google Scholar 

  6. 6

    Woolf, D., Amonette, J. E., Street–Perrott, F. A., Lehmann, J. & Joseph, S. Sustainable biochar to mitigate global climate change. Nat. Commun. 1, 56 (2010).

    Article  CAS  Google Scholar 

  7. 7

    Singh, B. P. & Cowie, A. L. Long-term influence of biochar on native organic carbon mineralisation in a low-carbon clayey soil. Sci. Rep. 4, 3687 (2014).

    Article  CAS  Google Scholar 

  8. 8

    Zimmerman, A. R. & Gao, B. in Biochar and Soil Biota (eds Ladygina, N. & Rineau, F.) 1–40 (CRC Press, 2013).

    Google Scholar 

  9. 9

    Van Zwieten, L. et al. Biochar for Environmental Management: Science, Technology and Implementation 2nd edn, 489–5209 (Routledge, 2015).

    Google Scholar 

  10. 10

    Novak, J. M. et al. Short–term CO2 mineralization after additions of biochar and switchgrass to a Typic Kandiudult. Geoderma 154, 281–288 (2010).

    CAS  Article  Google Scholar 

  11. 11

    Van Zwieten, L. et al. Effects of biochar from slow pyrolysis of papermill waste on agronomic performance and soil fertility. Plant Soil 327, 235–246 (2010).

    CAS  Article  Google Scholar 

  12. 12

    Keith, A., Singh, B. & Dijkstra, F. A. Biochar reduces the rhizosphere priming effect on soil organic carbon. Soil Biol. Biochem. 88, 372–379 (2015).

    CAS  Article  Google Scholar 

  13. 13

    Weng, Z. et al. Plant–biochar interactions drive the negative priming of soil organic carbon in an annual ryegrass field system. Soil Biol. Biochem. 90, 111–121 (2015).

    CAS  Article  Google Scholar 

  14. 14

    Whitman, T., Enders, A. & Lehmann, J. Pyrogenic carbon additions to soil counteract positive priming of soil carbon mineralization by plants. Soil Biol. Biochem. 73, 33–41 (2014).

    CAS  Article  Google Scholar 

  15. 15

    Maestrini, B., Nannipieri, P. & Abiven, S. A meta-analysis on pyrogenic organic matter induced priming effect. Glob. Change Biol. Bioenergy 7, 577–590 (2014).

    Article  CAS  Google Scholar 

  16. 16

    Luo, Y., Durenkamp, M., De Nobili, M., Lin, Q. & Brookes, P. C. Short term soil priming effects and the mineralisation of biochar following its incorporation to soils of different pH. Soil Biol. Biochem. 43, 2304–2314 (2011).

    CAS  Article  Google Scholar 

  17. 17

    Kuzyakov, Y., Subbotina, I., Chen, H., Bogomolova, I. & Xu, X. Black carbon decomposition and incorporation into soil microbial biomass estimated by 14C labeling. Soil Biol. Biochem. 41, 210–219 (2009).

    CAS  Article  Google Scholar 

  18. 18

    Six, J., Elliott, E. T., Paustian, K. & Doran, J. W. Aggregation and soil organic matter accumulation in cultivated and native grassland soils. Soil Sci. Soc. Am. J. 62, 1367–1377 (1998).

    CAS  Article  Google Scholar 

  19. 19

    Kong, A. Y., Six, J., Bryant, D. C., Denison, R. F. & Van Kessel, C. The relationship between carbon input, aggregation, and soil organic carbon stabilization in sustainable cropping systems. Soil Sci. Soc. Am. J. 69, 1078–1085 (2005).

    CAS  Article  Google Scholar 

  20. 20

    Kleber, M. et al. Chapter one–Mineral–organic associations: formation, properties, and relevance in soil environments. Adv. Agron. 130, 1–140 (2015).

    Article  Google Scholar 

  21. 21

    Torn, M. S., Trumbore, S. E., Chadwick, O. A., Vitousek, P. M. & Hendricks, D. M. Mineral control of soil organic carbon storage and turnover. Nature 389, 170–173 (1997).

    CAS  Article  Google Scholar 

  22. 22

    Mikutta, R., Kleber, M. & Jahn, R. Poorly crystalline minerals protect organic carbon in clay subfractions from acid subsoil horizons. Geoderma 128, 106–115 (2005).

    CAS  Article  Google Scholar 

  23. 23

    Denef, K., Plante, A. & Six, J. Soil Carbon Dynamics: An Integrated Methodology 91–126 (Cambridge Univ. Press, 2009).

    Google Scholar 

  24. 24

    Singh, B. P., Cowie, A. L. & Smernik, R. J. Biochar carbon stability in a clayey soil as a function of feedstock and pyrolysis temperature. Environ. Sci. Technol. 46, 11770–11778 (2012).

    CAS  Article  Google Scholar 

  25. 25

    Joseph, S. D. et al. An investigation into the reactions of biochar in soil. Aust. J. Soil Res. 48, 501–515 (2010).

    CAS  Article  Google Scholar 

  26. 26

    Keiluweit, M. et al. Mineral protection of soil carbon counteracted by root exudates. Nat. Clim. Change 5, 588–595 (2015).

    CAS  Article  Google Scholar 

  27. 27

    Haichar, F. e. Z., Santaella, C., Heulin, T. & Achouak, W. Root exudates mediated interactions belowground. Soil Biol. Biochem. 77, 69–80 (2014).

    CAS  Article  Google Scholar 

  28. 28

    Ventura, M. et al. Effect of biochar addition on soil respiration partitioning and root dynamics in an apple orchard. Eur. J. Soil Sci. 65, 186–195 (2014).

    CAS  Article  Google Scholar 

  29. 29

    Whitman, T., Singh, B. P. & Zimmerman, A. Biochar for Environmental Management: Science, Technology and Implementation 2nd edn, 455–488 (Routledge, 2015).

    Google Scholar 

  30. 30

    Slavich, P. G. et al. Contrasting effects of manure and green waste biochars on the properties of an acidic ferralsol and productivity of a subtropical pasture. Plant Soil 366, 213–227 (2013).

    CAS  Article  Google Scholar 

  31. 31

    Farquhar, G. D., Ehleringer, J. R. & Hubick, K. T. Carbon isotope discrimination and photosynthesis. Annu. Rev. Plant Biol. 40, 503–537 (1989).

    CAS  Article  Google Scholar 

  32. 32

    Lehmann, J. et al. Biochar effects on soil biota—a review. Soil Biol. Biochem. 43, 1812–1836 (2011).

    CAS  Article  Google Scholar 

  33. 33

    Liang, B. et al. Black carbon affects the cycling of non-black carbon in soil. Organ. Geochem. 41, 206–213 (2010).

    CAS  Article  Google Scholar 

  34. 34

    Lawrinenko, M. & Laird, D. A. Anion exchange capacity of biochar. Green Chem. 17, 4628–4636 (2015).

    CAS  Google Scholar 

  35. 35

    Desjardins, T., Barros, E., Sarrazin, M., Girardin, C. & Mariotti, A. Effects of forest conversion to pasture on soil carbon content and dynamics in Brazilian Amazonia. Agric. Ecosyst. Environ. 103, 365–373 (2004).

    CAS  Article  Google Scholar 

  36. 36

    Solomon, D. et al. Micro- and nano-environments of carbon sequestration: Multi-element STXM–NEXAFS spectromicroscopy assessment of microbial carbon and mineral associations. Chem. Geol. 329, 53–73 (2012).

    CAS  Article  Google Scholar 

  37. 37

    Solomon, D. et al. Molecular signature and sources of biochemical recalcitrance of organic C in Amazonian Dark Earths. Geochim. Cosmochim. Acta 71, 2285–2298 (2007).

    CAS  Article  Google Scholar 

  38. 38

    Heymann, K., Lehmann, J., Solomon, D., Schmidt, M. W. & Regier, T. C 1s K–edge near edge X–ray absorption fine structure (NEXAFS) spectroscopy for characterizing functional group chemistry of black carbon. Organ. Geochem. 42, 1055–1064 (2011).

    CAS  Article  Google Scholar 

  39. 39

    Solomon, D., Lehmann, J., Kinyangi, J., Liang, B. & Schäfer, T. Carbon K–edge NEXAFS and FTIR–ATR spectroscopic investigation of organic carbon speciation in soils. Soil Sci. Soc. Am. J. 69, 107–119 (2005).

    CAS  Article  Google Scholar 

  40. 40

    Rutherford, D. W., Wershaw, R. L., Rostad, C. E. & Kelly, C. N. Effect of formation conditions on biochars: compositional and structural properties of cellulose, lignin, and pine biochars. Biomass Bioenergy 46, 693–701 (2012).

    CAS  Article  Google Scholar 

  41. 41

    Artz, R. R. et al. FTIR spectroscopy can be used as a screening tool for organic matter quality in regenerating cutover peatlands. Soil Biol. Biochem. 40, 515–527 (2008).

    CAS  Article  Google Scholar 

  42. 42

    Mouvenchery, Y. K., Kučerí, J., Diehl, D. & Schaumann, G. E. Cation-mediated cross-linking in natural organic matter: a review. Rev. Environ. Sci. Biotechnol. 11, 41–54 (2012).

    Article  Google Scholar 

  43. 43

    Archanjo, B. S. et al. Chemical analysis and molecular models for calcium–oxygen–carbon interactions in black carbon found in fertile Amazonian anthrosoils. Environ. Sci. Technol. 48, 7445–7452 (2014).

    CAS  Article  Google Scholar 

  44. 44

    Araujo, J. R. et al. Selective extraction of humic acids from an anthropogenic Amazonian dark earth and from a chemically oxidized charcoal. Biol. Fertil. Soils 50, 1223–1232 (2014).

    CAS  Article  Google Scholar 

  45. 45

    Cheng, C.-H., Lehmann, J., Thies, J. E., Burton, S. D. & Engelhard, M. H. Oxidation of black carbon by biotic and abiotic processes. Organ. Geochem. 37, 1477–1488 (2006).

    CAS  Article  Google Scholar 

  46. 46

    Nguyen, B. T. et al. Long-term black carbon dynamics in cultivated soil. Biogeochemistry 89, 295–308 (2008).

    CAS  Article  Google Scholar 

  47. 47

    Lehmann, J. et al. Near-edge X-ray absorption fine structure (NEXAFS) spectroscopy for mapping nano-scale distribution of organic carbon forms in soil: application to black carbon particles. Glob. Biogeochem. Cycles 19, GB1013 (2005).

    Google Scholar 

  48. 48

    Pietikäinen, J., Kiikkilä, O. & Fritze, H. Charcoal as a habitat for microbes and its effect on the microbial community of the underlying humus. Oikos 89, 231–242 (2000).

    Article  Google Scholar 

  49. 49

    Hafner, S. et al. Effect of grazing on carbon stocks and assimilate partitioning in a Tibetan montane pasture revealed by 13CO2 pulse labeling. Glob. Change Biol. 18, 528–538 (2012).

    Article  Google Scholar 

  50. 50

    Singh, B. P. et al. In situ persistence and migration of biochar carbon and its impact on native carbon emission in contrasting soils under managed temperate pastures. PLoS ONE 10, e0141560 (2015).

    Article  CAS  Google Scholar 

  51. 51

    IPCC Climate Change 2014: Synthesis Report (eds Core Writing Team, Pachauri, R. K. & Meyer L. A.) (IPCC, 2015).

  52. 52

    Lal, R. Soil carbon sequestration to mitigate climate change. Geoderma 123, 1–22 (2004).

    CAS  Article  Google Scholar 

  53. 53

    Lehmann, J., Gaunt, J. & Rondon, M. Bio-char sequestration in terrestrial ecosystems—a review. Mitig. Adapt. Strat. Glob. Change 11, 395–419 (2006).

    Article  Google Scholar 

  54. 54

    Fang, Y., Singh, B. & Singh, B. P. Effect of temperature on biochar priming effects and its stability in soils. Soil Biol. Biochem. 80, 136–145 (2015).

    CAS  Article  Google Scholar 

  55. 55

    Derrien, D. et al. Does the addition of labile substrate destabilise old soil organic matter? Soil Biol. Biochem. 76, 149–160 (2014).

    CAS  Article  Google Scholar 

  56. 56

    Gunina, A. & Kuzyakov, Y. Pathways of litter C by formation of aggregates and SUM density fractions: implications from 13C natural abundance. Soil Biol. Biochem. 71, 95–104 (2014).

    CAS  Article  Google Scholar 

  57. 57

    Six, J., Elliott, E. T., Paustian, K. & Doran, J. W. Aggregation and soil organic matter accumulation in cultivated and native grassland soils. Soil Sci. Soc. Am. J. 62, 1367–1377 (1998).

    CAS  Article  Google Scholar 

  58. 58

    Campbell, C. D., Chapman, S. J., Cameron, C. M., Davidson, M. S. & Potts, J. M. A rapid microtiter plate method to measure carbon dioxide evolved from carbon substrate amendments so as to determine the physiological profiles of soil microbial communities by using whole soil. Appl. Environ. Microbiol. 69, 3593–3599 (2003).

    CAS  Article  Google Scholar 

  59. 59

    Marx, M-C., Wood, M. & Jarvis, S. A microplate fluorimetric assay for the study of enzyme diversity in soils. Soil Biol. Biochem. 33, 1633–1640 (2001).

    CAS  Article  Google Scholar 

  60. 60

    Turner, B. L. Variation in pH optima of hydrolytic enzyme activities in tropical rain forest soils. Appl. Environ. Microbiol. 76, 6485–6493 (2010).

    CAS  Article  Google Scholar 

  61. 61

    R Development Core Team R: A Language and Environment for Statistical Computing (R Foundation for Statistical Computing, 2012); http://www.R-project.org

  62. 62

    Butler, T., Dawson, C. & Wildey, T. A posteriori error analysis of stochastic differential equations using polynomial chaos expansions. Siam J. Sci. Comput. 33, 1267–1291 (2011).

    Article  Google Scholar 

  63. 63

    Jirka, S. & Tomlinson, T. 2013 State of the Biochar Industry: A Survey of Commercial Activity in the Biochar Field (International Biochar Initiative, 2013); http://www.biochar-international.org/sites/default/files/State_of_the_Biochar_Industry_2013.pdf

  64. 64

    Stöhr, J. NEXAFS spectroscopy Vol. 25 (Springer Science & Business Media, 2013).

    Google Scholar 

Download references

Acknowledgements

The authors thank the Australian Government, Department of Agriculture and Water Resources for supporting the National Biochar Initiatives (2009–2012, 2012–2014) which co-funded this research. We are particularly grateful to P. Slavich, as one of the key founders of this long-term field experiment, for providing insightful comments on the initial draft. Part of this research was undertaken on the soft X-ray spectroscopy beamline at the Australian Synchrotron, Victoria, Australia (grant number AS151_SXR_9599). We thank the chief beamline scientist, B. Cowie, for his technical support on the soft X-ray analysis. We also appreciate the technical support from S. Petty and J. Rust for maintaining this field experiment over the past decade, and laboratory support from N. Morris. We also thank C. Achete from INMETRO, Brazil and B. Gong from the University of New South Wales, Australia, for performing XPS analysis of biochars and soils, respectively. We acknowledge the intellectual contribution from S. Donne for discussions on the potential mechanisms of biochar-induced stabilization of rhizodeposits. We also thank Y. Fang for giving advice and reviewing 13C calculations.

Author information

Affiliations

Authors

Contributions

Z.W. drafted and wrote the manuscript, carried out experimental design, setup and conducted experiments, data collection and analysis; L.V.Z. and B.P.S. wrote the manuscript, aided in experimental design and provided critical revision of the article; E.T. aided in experimental design, data collection and analysis, and provided critical revision of the article; S.J. and M.T.R. collected and analysed data, and provided critical revision of the article; L.M.M. wrote the manuscript, and provided critical revision of the article; T.J.R. and S.W.L.K. provided critical revision of the article; S.M. and D.C. analysed data and provided critical revision of the article; J.R.A. analysed data; B.S.A. and A.C. provided critical revision of the article. All authors provided final approval of the revision to be published.

Corresponding author

Correspondence to Lukas Van Zwieten.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Information

Supplementary Information (PDF 1012 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

(Han) Weng, Z., Van Zwieten, L., Singh, B. et al. Biochar built soil carbon over a decade by stabilizing rhizodeposits. Nature Clim Change 7, 371–376 (2017). https://doi.org/10.1038/nclimate3276

Download citation

Further reading