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The tRNA synthetase paralog PoxA modifies elongation factor-P with (R)-β-lysine

Nature Chemical Biology volume 7, pages 667669 (2011) | Download Citation

Abstract

The lysyl-tRNA synthetase paralog PoxA modifies elongation factor P (EF-P) with α-lysine at low efficiency. Cell-free extracts containing non–α-lysine substrates of PoxA modified EF-P with a change in mass consistent with addition of β-lysine, a substrate also predicted by genomic analyses. EF-P was efficiently functionally modified with (R)-β-lysine but not (S)-β-lysine or genetically encoded α-amino acids, indicating that PoxA has evolved an activity orthogonal to that of the canonical aminoacyl-tRNA synthetases.

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Acknowledgements

We would like to thank Michael Thomas (University of Wisconsin) for the generous gift of (S)-β-lysine. This work was supported by the US National Institutes of Health (GM65183, M.I.), the Canada Institutes for Health Research (MOP-86683 and MSH-87729, W.W.N.) and the Natural Sciences and Engineering Research Council of Canada (RGPIN 386286-10, W.W.N.; Vanier Graduate Scholarship, S.B.Z). We also acknowledge The Ohio State University for partial financial support.

Author information

Author notes

    • Hervé Roy

    Present address: Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, Florida, USA.

Affiliations

  1. Department of Microbiology, Center for RNA Biology, Ohio State University, Columbus, Ohio, USA.

    • Hervé Roy
    • , Tammy J Bullwinkle
    •  & Michael Ibba
  2. Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.

    • S Betty Zou
    •  & William W Navarre
  3. Department of Chemistry, Ohio State University, Columbus, Ohio, USA.

    • Benjamin S Wolfe
    •  & Craig J Forsyth
  4. Ohio State Biochemistry Program, Ohio State University, Columbus, Ohio, USA.

    • Marla S Gilreath
    •  & Michael Ibba

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Contributions

H.R., S.B.Z., T.J.B., B.S.W. and M.S.G. performed experiments and analyzed the resulting data; H.R., C.J.F., W.W.N. and M.I. designed experiments and wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Michael Ibba.

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    Supplementary Figures 1–5, Supplementary Methods and Supplementary Results

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DOI

https://doi.org/10.1038/nchembio.632

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