Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Enzymology

Radical break-up, blissful make-up

The radical SAM superfamily of enzymes provides, yet again, fertile ground for the discovery of amazing new biochemical transformations. Strong evidence is now presented for an unprecedented radical fragmentation-recombination of L-tryptophan to a derivative that is incorporated into the complex antibiotic nosiheptide, highlighting the versatility of radical mechanisms for complex biochemical reactions.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

Figure 1: Scheme showing the NosL-catalyzed transformation of L-tryptophan to MIA and its incorporation into nosiheptide.

References

  1. Sofia, H.J., Chen, G., Hetzler, B.G., Reyes-Spindola, J.F. & Miller, N.E. Nucleic Acids Res. 29, 1097–1106 (2001).

    Article  CAS  Google Scholar 

  2. Frey, P.A., Hegeman, A.D. & Ruzicka, F.J. Crit. Rev. Biochem. Mol. Biol. 43, 63–88 (2008).

    Article  CAS  Google Scholar 

  3. Chatterjee, A. et al. Nat. Chem. Biol. 4, 758–765 (2008).

    Article  CAS  Google Scholar 

  4. Martinez-Gomez, N.C. & Downs, D.M. Biochemistry 47, 9054–9056 (2008).

    Article  CAS  Google Scholar 

  5. Zhang, Q. et al. Nat. Chem. Biol. 7, 154–160 (2011).

    Article  CAS  Google Scholar 

  6. Hänzelmann, P. & Schindelin, H. Proc. Natl. Acad. Sci. USA 101, 12870–12875 (2004).

    Article  Google Scholar 

  7. McCarty, R.M., Somogyi, Á., Lin, G., Jacobsen, N.E. & Bandarian, V. Biochemistry 48, 3847–3852.

  8. Yu, Y. et al. ACS Chem. Biol. 4, 855–864 (2009).

    Article  CAS  Google Scholar 

  9. Kriek, M., Martins, F., Challand, M.R., Croft, A. & Roach, P.L. Angew. Chem. Int. Edn. Engl. 46, 9223–9226 (2007).

    Article  CAS  Google Scholar 

  10. Driesener, R.C. et al. Angew. Chem. Int. Edn. Engl. 49, 1687–1690 (2010).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Squire J Booker.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Arcinas, A., Booker, S. Radical break-up, blissful make-up. Nat Chem Biol 7, 133–134 (2011). https://doi.org/10.1038/nchembio.528

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nchembio.528

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing