Monoubiquitylation of histone H2B is found to disrupt condensation of chemically defined chromatin fibers. A novel fluorescence-based assay is used in concert with analytical ultracentrifugation to uncover the synergistic roles of histone acetylation and ubiquitylation on chromatin dynamics.
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References
Fierz, B. et al. Nat. Chem. Biol. 7, 113–119 (2011).
Sun, Z.W. & Allis, C.D. Nature 418, 104–108 (2002).
Chernikova, S.B., Dorth, J.A., Razorenova, O.V., Game, J.C. & Brown, J.M. Radiat. Res. 174, 558–565 (2010).
Chatterjee, C. & Muir, T.W. J. Biol. Chem. 285, 11045–11050 (2010).
McGinty, R.K., Kim, J., Chatterjee, C., Roeder, R.G. & Muir, T.W. Nature 453, 812–816 (2008).
Chatterjee, C., McGinty, R.K., Fierz, B. & Muir, T.W. Nat. Chem. Biol. 6, 267–269 (2010).
Shogren-Knaak, M. et al. Science 311, 844–847 (2006).
Lu, X. et al. Nat. Struct. Mol. Biol. 15, 1122–1124 (2008).
Watanabe, S. et al. Biochim. Biophys. Acta 1799, 480–486 (2010).
Kim, J. et al. Cell 137, 459–471 (2009).
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Peterson, C. A ubiquitin crowbar opens chromatin. Nat Chem Biol 7, 68–69 (2011). https://doi.org/10.1038/nchembio.514
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DOI: https://doi.org/10.1038/nchembio.514
