Flap endonuclease 1 (FEN1), a structure-specific endo- and exonuclease, has multiple functions that determine essential biological processes, such as cell proliferation and cell death. As such, the enzyme must be precisely regulated to execute each of its functions with the right timing and in a specific subcellular location. Here we report that FEN1 is methylated at arginine residues, primarily at Arg192. The methylation suppresses FEN1 phosphorylation at Ser187. The methylated form, but not the phosphorylated form, of FEN1 strongly interacts with proliferating cell nuclear antigen (PCNA), ensuring the 'on' and 'off' timing of its reaction. Mutations of FEN1 disrupting arginine methylation and PCNA interaction result in unscheduled phosphorylation and a failure to localize to DNA replication or repair foci. This consequently leads to a defect in Okazaki fragment maturation, a delay in cell cycle progression, impairment of DNA repair and a high frequency of genome-wide mutations.
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We thank G. Tsaprailis in the Proteomics Core facility of University of Arizona for technical assistance in determining the methylation sites on FEN1. We thank S.R. da Costa for editorial assistance. This work was supported by US National Institutes of Health grants RO1 CA073764 and R01CA085344 to B.S.
The authors declare no competing financial interests.
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Guo, Z., Zheng, L., Xu, H. et al. Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding. Nat Chem Biol 6, 766–773 (2010). https://doi.org/10.1038/nchembio.422
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