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High-content screens

Giving Rho(d) directions

Drugs identified in high-content screens are often difficult to link to the cellular target, especially when multiple signaling pathways impinge on the phenotypic endpoint. A chemical-genetic approach in fruit fly cells now greatly improves the prioritization of drug hits by directing the screen toward a single pathway.

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Figure 1: Sensitizer screen for Rho pathway inhibitors.

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Correspondence to Sebastian M B Nijman.

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The authors declare no competing financial interests.

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Muellner, M., Nijman, S. Giving Rho(d) directions. Nat Chem Biol 6, 397–398 (2010). https://doi.org/10.1038/nchembio.373

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