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The art of the chemical probe

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Chemical biologists frequently aim to create small-molecule probes that interact with a specific protein in vitro in order to explore the role of the protein in a broader biological context (cells or organisms), but a common understanding of what makes a high-quality probe is lacking. Here I propose a set of principles to guide probe qualification.

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Correspondence to Stephen V Frye.

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The author declares no competing financial interests.

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Frye, S. The art of the chemical probe. Nat Chem Biol 6, 159–161 (2010). https://doi.org/10.1038/nchembio.296

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