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The ABCs of PTMs

Post-translational modifications (PTMs) are ubiquitous in all forms of life and often modulate critical protein functions. Recent chemical and biological advances have finally enabled scientists to precisely modify proteins at physiologically relevant positions, ushering in a new era of protein studies.

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Figure 1: Methods for synthesizing recombinant proteins containing PTMs.
Figure 2: Basic applications of modified recombinant proteins.
Figure 3: The future of PTM studies.

References

  1. Hutchison, C.A. et al. Science 351, aad6253 (2016).

    Article  Google Scholar 

  2. Nielsen, A.A.K. et al. Science 352, aac7341 (2016).

    Article  Google Scholar 

  3. Boyken, S.E. et al. Science 352, 680–687 (2016).

    Article  CAS  Google Scholar 

  4. Bodenmiller, B., Mueller, L.N., Mueller, M., Domon, B. & Aebersold, R. Nat. Methods 4, 231–237 (2007).

    Article  CAS  Google Scholar 

  5. Muir, T.W., Sondhi, D. & Cole, P.A. Proc. Natl. Acad. Sci. USA 95, 6705–6710 (1998). CrossRef

    Article  CAS  Google Scholar 

  6. Macmillan, D., Bill, R.M., Sage, K.A., Fern, D. & Flitsch, S.L. Chem. Biol. 8, 133–145 (2001).

    Article  CAS  Google Scholar 

  7. Chin, J.W. Nature 550, 53–60 (2017).

    Article  CAS  Google Scholar 

  8. Liu, C.C. & Schultz, P.G. Annu. Rev. Biochem. 79, 413–444 (2010).

    Article  CAS  Google Scholar 

  9. Park, H.-S. et al. Science 333, 1151–1154 (2011).

    Article  CAS  Google Scholar 

  10. Rösner, D., Schneider, T., Schneider, D., Scheffner, M. & Marx, A. Nat. Protoc. 10, 1594–1611 (2015).

    Article  Google Scholar 

  11. Lajoie, M.J. et al. Science 342, 357–360 (2013).

    Article  CAS  Google Scholar 

  12. Italia, J.S. et al. Nat. Chem. Biol. 13, 446–450 (2017).

    Article  CAS  Google Scholar 

  13. Lajoie, M.J., Söll, D. & Church, G.M. J. Mol. Biol. 428 5 Pt B, 1004–1021 (2016).

    Article  CAS  Google Scholar 

  14. Pirman, N.L. et al. Nat. Commun. 6, 8130 (2015).

    Article  Google Scholar 

  15. Lammers, M., Neumann, H., Chin, J.W. & James, L.C. Nat. Chem. Biol. 6, 331–337 (2010).

    Article  CAS  Google Scholar 

  16. Sekar, T.V., Foygel, K., Gelovani, J.G. & Paulmurugan, R. Anal. Chem. 87, 892–899 (2015).

    Article  CAS  Google Scholar 

  17. Huang, X. & Dixit, V.M. Cell Res. 26, 484–498 (2016).

    Article  CAS  Google Scholar 

  18. Zhang, M.S. et al. Nat. Methods 14, 729–736 (2017).

    Article  CAS  Google Scholar 

  19. Ordureau, A. et al. Proc. Natl. Acad. Sci. USA 112, 6637–6642 (2015).

    Article  CAS  Google Scholar 

  20. Zhang, Y. et al. Nature 551, 644–647 (2017).

    Article  CAS  Google Scholar 

  21. Boeke, J.D. et al. Science 353, 126–127 (2016).

    Article  CAS  Google Scholar 

  22. Rogerson, D.T. et al. Nat. Chem. Biol. 11, 496–503 (2015).

    Article  CAS  Google Scholar 

  23. Luo, X. et al. Nat. Chem. Biol. 13, 845–849 (2017).

    Article  CAS  Google Scholar 

  24. Meuris, L. et al. Nat. Biotechnol. 32, 485–489 (2014).

    Article  CAS  Google Scholar 

  25. Dann, G.P. et al. Nature 548, 607–611 (2017).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

K.W.B. acknowledges support from the NSF (DGE-1122492), and J.R. is supported by the NIH (R01GM125951; R01GM117230; P01DK017433; U54CA209992).

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Correspondence to Jesse Rinehart.

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Barber, K., Rinehart, J. The ABCs of PTMs. Nat Chem Biol 14, 188–192 (2018). https://doi.org/10.1038/nchembio.2572

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