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Protein engineering

Finding the best ligase

Nature Chemical Biology volume 14, pages 23 (2018) | Download Citation

Modification of folded proteins at will, within any sequence context, remains an elusive goal. A proteome-wide screening approach has now identified a set of protein ligases that enables conjugation of peptides to almost any protein N terminus, overcoming longstanding limitations in protein engineering.

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Author information

Affiliations

  1. Christian F.W. Becker is in the Institute of Biological Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria.

    • Christian F W Becker

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Competing interests

The author declares no competing financial interests.

Corresponding author

Correspondence to Christian F W Becker.

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DOI

https://doi.org/10.1038/nchembio.2533

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