Abstract
Metabolic exchange between an organism and the environment, including interactions with neighboring organisms, is important for processes of organismal development. Here we develop and use thin-layer agar natural product MALDI-TOF imaging mass spectrometry of intact bacterial colonies grown on top of the MALDI target plate to study an interaction between two species of bacteria and provide direct evidence that Bacillus subtilis silences the defensive arsenal of Streptomyces coelicolor.
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Acknowledgements
The authors acknowledge M. Burkart (University of California, San Diego) for S. coelicolor A3(2). The funding was provided by the Beckman Foundation, the annotation of the cyclic peptides in this study was supported by NIHGM086283 (P.C.D.) and P.S. acknowledges financial support from Texas A&M University.
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Y.-L.Y., Y.X. and P.C.D. prepared bacterial cultures and performed IMS. Y.-L.Y., Y.X., P.S. and P.C.D. were involved in the data analysis and interpretation. P.S. prepared the mutant strains. Y.-L.Y., P.S. and P.C.D. wrote the paper.
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Yang, YL., Xu, Y., Straight, P. et al. Translating metabolic exchange with imaging mass spectrometry. Nat Chem Biol 5, 885–887 (2009). https://doi.org/10.1038/nchembio.252
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DOI: https://doi.org/10.1038/nchembio.252
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