Brief Communication | Published:

Translating metabolic exchange with imaging mass spectrometry

Nature Chemical Biology volume 5, pages 885887 (2009) | Download Citation

Abstract

Metabolic exchange between an organism and the environment, including interactions with neighboring organisms, is important for processes of organismal development. Here we develop and use thin-layer agar natural product MALDI-TOF imaging mass spectrometry of intact bacterial colonies grown on top of the MALDI target plate to study an interaction between two species of bacteria and provide direct evidence that Bacillus subtilis silences the defensive arsenal of Streptomyces coelicolor.

  • Compound C16H18N2O4S

    Penicillin G

  • Compound C51H89N7O13

    Surfactin-C13

  • Compound C52H91N7O13

    Surfactin-C14

  • Compound C53H93N7O13

    Surfactin-C15

  • Compound C73H111N11O20

    Plipastatin-C16-Ala

  • Compound C74H112N10O21

    Plipastatin-C17-Ala

  • Compound C75H114N10O21

    Plipastatin-C16-Val

  • Compound C76H117N11O20

    Plipastatin-C17-Val

  • Compound C152H226N38O45S3

    Subtilosin

  • Compound C34H50N2O6

    Bacillaene

  • Compound C25H33N3O

    Streptorubin B

  • Compound C25H35N3O

    Undecylprodiginine

  • Compound C12H18N2O5

    Bacilysin

  • Compound C84H139N25O29S2

    SapB

  • Compound C67H78N14O26

    CDA4a

  • Compound C67H80N14O26

    CDA4b

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References

  1. 1.

    & Curr. Opin. Microbiol. 12, 205–214 (2009).

  2. 2.

    Appl. Microbiol. Biotechnol. 75, 267–277 (2007).

  3. 3.

    & Proc. Natl. Acad. Sci. USA 105, 18126–18131 (2008).

  4. 4.

    , , & Nat. Methods 4, 828–833 (2007).

  5. 5.

    et al. Mol. Biosyst. 4, 562–570 (2008).

  6. 6.

    et al. Proc. Natl. Acad. Sci. USA 105, 4587–4594 (2008).

  7. 7.

    , , , & Rapid Commun. Mass Spectrom. 13, 943–949 (1999).

  8. 8.

    , & Int. J. Mass Spectrom. 268, 254–264 (2007).

  9. 9.

    , , & J. Biochem. 98, 585–603 (1985).

  10. 10.

    Rapid Commun. Mass Spectrom. 22, 1146–1152 (2008).

  11. 11.

    et al. Anal. Chem. 80, 1942–1951 (2008).

  12. 12.

    et al. J. Am. Soc. Mass Spectrom. 19, 1856–1866 (2008).

  13. 13.

    et al. J. Am. Chem. Soc. 128, 14754–14755 (2006).

  14. 14.

    , , & Nat. Chem. Biol. 1, 265–269 (2005).

  15. 15.

    & J. Gen. Microbiol. 96, 289–297 (1976).

  16. 16.

    et al. Proc. Natl. Acad. Sci. USA 101, 11448–11453 (2004).

  17. 17.

    , & Biochemistry 43, 2915–2925 (2004).

  18. 18.

    , , , & Proc. Natl. Acad. Sci. USA 105, 17510–17515 (2008).

  19. 19.

    , , , & Proc. Natl. Acad. Sci. USA 98, 11621–11626 (2001).

  20. 20.

    et al. Anal. Chem. 81, 4200–4209 (2009).

  21. 21.

    et al. Nat. Methods 6, 596–599 (2009).

  22. 22.

    , , , & Proc. Natl. Acad. Sci. USA 104, 305–310 (2007).

  23. 23.

    et al. Proc. Natl. Acad. Sci. USA 104, 1506–1509 (2007).

  24. 24.

    et al. Biochemistry 37, 1585–1595 (1998).

  25. 25.

    , & J. Bacteriol. 188, 4918–4925 (2006).

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Acknowledgements

The authors acknowledge M. Burkart (University of California, San Diego) for S. coelicolor A3(2). The funding was provided by the Beckman Foundation, the annotation of the cyclic peptides in this study was supported by NIHGM086283 (P.C.D.) and P.S. acknowledges financial support from Texas A&M University.

Author information

Author notes

    • Yu-Liang Yang
    •  & Yuquan Xu

    These authors contributed equally to this work.

Affiliations

  1. Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, USA.

    • Yu-Liang Yang
    • , Yuquan Xu
    •  & Pieter C Dorrestein
  2. Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

    • Paul Straight
  3. Department of Pharmacology and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA.

    • Pieter C Dorrestein
  4. Center for Marine Biotechnology and Biomedicine, University of California, San Diego, La Jolla, California, USA.

    • Pieter C Dorrestein

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Contributions

Y.-L.Y., Y.X. and P.C.D. prepared bacterial cultures and performed IMS. Y.-L.Y., Y.X., P.S. and P.C.D. were involved in the data analysis and interpretation. P.S. prepared the mutant strains. Y.-L.Y., P.S. and P.C.D. wrote the paper.

Corresponding authors

Correspondence to Paul Straight or Pieter C Dorrestein.

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DOI

https://doi.org/10.1038/nchembio.252

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