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Genetic code expansion

Synthetases pick up the PACE

Phage-assisted evolution can rapidly improve the efficiency and substrate specificity of orthogonal aminoacyl-tRNA synthetases. Furthermore, the crystal structure of the pyrrolysyl-tRNA synthetase N-terminal domain reveals the basis for these improvements and provides a structural rationale for orthogonality.

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Figure 1: Phage display accelerates the evolution of orthogonal aaRSs.

Debbie Maizels/Springer Nature


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Correspondence to Wenshe R Liu.

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Tharp, J., Liu, W. Synthetases pick up the PACE. Nat Chem Biol 13, 1205–1206 (2017).

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