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Genetic code expansion

Synthetases pick up the PACE

Nature Chemical Biology volume 13, pages 12051206 (2017) | Download Citation

Phage-assisted evolution can rapidly improve the efficiency and substrate specificity of orthogonal aminoacyl-tRNA synthetases. Furthermore, the crystal structure of the pyrrolysyl-tRNA synthetase N-terminal domain reveals the basis for these improvements and provides a structural rationale for orthogonality.

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Author information

Affiliations

  1. Jeffery M. Tharp and Wenshe R. Liu are in the Department of Chemistry, Texas A&M University, College Station, Texas, USA.

    • Jeffery M Tharp
    •  & Wenshe R Liu

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Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Wenshe R Liu.

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DOI

https://doi.org/10.1038/nchembio.2516

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