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Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells

Nature Chemical Biology volume 13, pages 839841 (2017) | Download Citation

Abstract

Cpf1 is a CRISPR effector protein that has greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a single RNA polymerase II–driven RNA transcript expressed in mammalian cells. This property simplifies modification of multiple genomic targets and can be used to increase the efficiency of Cpf1-mediated editing.

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Acknowledgements

This work is supported by NIH R37 AI091476 and P01 AI100263 (M.F.).

Author information

Affiliations

  1. Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, Florida, USA.

    • Guocai Zhong
    • , Haimin Wang
    • , Yujun Li
    • , Mai H Tran
    •  & Michael Farzan
  2. Department of Microbiology and Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.

    • Yujun Li

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Contributions

G.Z. conceived this study and developed it with assistance from M.F.; G.Z., H.W., Y.L., and M.H.T. performed all experiments; M.F. and G.Z. wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Guocai Zhong.

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    Supplementary Text and Figures

    Supplementary Results, Supplementary Tables 1–4 and Supplementary Figures 1–6

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DOI

https://doi.org/10.1038/nchembio.2410

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