Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Target engagement

Shining a light

The ability to measure the binding of a compound to its intended target in live cells or tissue is a critical parameter for drug discovery. A new method using polarized light microscopy adds to the current toolbox by enabling monitoring of target engagement in vitro and in vivo at single-cell resolution.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Prices vary by article type



Prices may be subject to local taxes which are calculated during checkout

Figure 1: Measuring cellular target engagement of unlabeled drugs by using fluorescence polarization microscopy in combination with companion imaging probes (CIPs).


  1. Bunnage, M.E., Chekler, E.L.P. & Jones, L.H. Nat. Chem. Biol. 9, 195–199 (2013).

    Article  CAS  Google Scholar 

  2. Cook, D. et al. Nat. Rev. Drug Discov. 13, 419–431 (2014).

    Article  CAS  Google Scholar 

  3. Dubach, J.M. Nat. Chem. Biol. 13, 168–173 (2017).

    Article  CAS  Google Scholar 

  4. Schürmann, M., Janning, P., Ziegler, S. & Waldmann, H. Cell Chem. Biol. 23, 435–441 (2016).

    Article  Google Scholar 

  5. Martinez Molina, D. et al. Science 341, 84–87 (2013).

    Article  Google Scholar 

  6. Reinhard, F.B.M. et al. Nat. Methods 12, 1129–1131 (2015).

    Article  CAS  Google Scholar 

  7. Huber, K.V.M. et al. Nat. Methods 12, 1055–1057 (2015).

    Article  CAS  Google Scholar 

  8. Savitski, M.M. et al. Science 346, 1255784 (2014).

    Article  Google Scholar 

  9. Snijder, B. et al. Nature 461, 520–523 (2009).

    Article  CAS  Google Scholar 

  10. Thurber, G.M. et al. Nat. Commun. 4, 1504 (2013).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Kilian V M Huber.

Ethics declarations

Competing interests

The author declares no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Huber, K. Shining a light. Nat Chem Biol 13, 133–134 (2017).

Download citation

  • Published:

  • Issue Date:

  • DOI:

This article is cited by


Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research