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A fluorescent probe for cysteine depalmitoylation reveals dynamic APT signaling

Nature Chemical Biology volume 13, pages 150–152 (2017)Cite this article

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Abstract

Hundreds of human proteins are modified by reversible palmitoylation of cysteine residues (S-palmitoylation), but the regulation of depalmitoylation is poorly understood. Here, we develop 'depalmitoylation probes' (DPPs), small-molecule fluorophores, to monitor the endogenous activity levels of 'erasers' of S-palmitoylation, acylprotein thioesterases (APTs). Live-cell analysis with DPPs reveals rapid growth-factor-mediated inhibition of the depalmitoylation activity of APTs, exposing a novel regulatory mechanism of dynamic lipid signaling.

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Figure 1: Design and in vitro validation of DPPs.
Figure 2: Analysis of endogenous and growth-factor-stimulated depalmitoylase activities using DPPs.

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Acknowledgements

This work was supported by the University of Chicago, the National Institute of General Medical Sciences of the National Institutes of Health (R35 GM119840) to B.C.D., the University of Chicago Medicine Comprehensive Cancer Center (P30CA14599), and a “Catalyst Award” to B.C.D. from the Chicago Biomedical Consortium, with support from the Searle Funds at The Chicago Community Trust. We thank J. Pu (University of Chicago) and D. Dammeier (University of Chicago) for technical assistance, and C. He (University of Chicago), A. Mukherje (National Institutes of Health), Y. Krishnan (University of Chicago), and J. Lewis (University of Chicago) for supplying materials and equipment.

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  1. Department of Chemistry, University of Chicago, Chicago, Illinois, USA

    Rahul S Kathayat, Pablo D Elvira & Bryan C Dickinson

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  1. Rahul S Kathayat
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  2. Pablo D Elvira
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  3. Bryan C Dickinson
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Contributions

R.S.K. and P.D.E. synthesized all compounds in the paper. R.S.K. performed all analytical measurements, in vitro assays, and cell culture experiments. R.S.K. and B.C.D. designed experimental strategies and wrote the paper.

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Correspondence to Bryan C Dickinson.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Results and Supplementary Figures 1–17. (PDF 4117 kb)

Supplementary Note

Synthetic Procedures. (PDF 1604 kb)

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Kathayat, R., Elvira, P. & Dickinson, B. A fluorescent probe for cysteine depalmitoylation reveals dynamic APT signaling. Nat Chem Biol 13, 150–152 (2017). https://doi.org/10.1038/nchembio.2262

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