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A multi-step peptidolytic cascade for amino acid recovery in chloroplasts

Nature Chemical Biology volume 13, pages 15–17 (2017)Cite this article

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Abstract

Plastids (including chloroplasts) are subcellular sites for a plethora of proteolytic reactions, required in functions ranging from protein biogenesis to quality control. Here we show that peptides generated from pre-protein maturation within chloroplasts of Arabidopsis thaliana are degraded to amino acids by a multi-step peptidolytic cascade consisting of oligopeptidases and aminopeptidases, effectively allowing the recovery of single amino acids within these organelles.

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Figure 1: Degradation of a chloroplastic targeting peptide to amino acids.
Figure 2: Analysis of peptide degradation to amino acids in stromal samples isolated from Arabidopsis Col-0, oligopeptidase and aminopeptidase mutants.

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Acknowledgements

We would like to thank the Swedish Metabolomics Centre (Umeå) for assistance with amino acid quantification, H. Fu (Academia Sinica) for Rpn10 antisera and U. Langel (Stockholm University) for the galanin peptide. This study was supported by grants from the Swedish Research Council to E.G. (DN2015-04833) and J.L. (DN2012-5145), from the Sigurd och Elsa Goljes Minne Foundation to P.F.T. and B.K., the ARC future fellowship to M.W.M., ARC Discovery program grant DP130102384 to J.W., Polish National Science Centre for Scientific Research grant NN401042838 to M.D. and the STINT Institutional grant for cooperation between the laboratories of E.G. and J.W.

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Author notes

  1. Pedro F Teixeira and Beata Kmiec: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Stockholm University, Arrhenius Laboratories for Natural Sciences, Stockholm, Sweden

    Pedro F Teixeira, Beata Kmiec & Elzbieta Glaser

  2. Department of Oncology-Pathology, Clinical Proteomics Mass Spectrometry, Science for Life Laboratory and Karolinska Institutet, Stockholm, Sweden

    Rui M M Branca & Janne Lehtiö

  3. ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, Western Australia, Australia

    Monika W Murcha & Aneta Ivanova

  4. Division of Bioorganic Chemistry, Department of Chemistry, Wroclaw University of Technology, Wroclaw, Poland

    Anna Byzia & Marcin Drag

  5. Department of Animal, ARC Centre of Excellence in Plant Energy Biology, Plant and Soil Science, School of Life Science, La Trobe University, Victoria, Australia

    James Whelan

Authors
  1. Pedro F Teixeira
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  8. Marcin Drag
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Contributions

P.F.T. designed the study and performed peptidase assays. B.K. selected and characterized Arabidopsis mutants and performed cellular fractionation experiments. R.M.M.B. carried out peptide MS analysis. M.W.M. performed protein localization experiments. A.B. carried out aminopeptidase profiling. P.F.T., B.K., R.M.M.B., A.B., M.W.M., A.I., J.W., M.D., J.L. and E.G. analyzed, discussed and interpreted the results. P.F.T. and B.K. wrote the paper with input from all coauthors.

Corresponding author

Correspondence to Pedro F Teixeira.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Text and Figures

Supplementary Results, Supplementary Figures 1–7 and Supplementary Tables 1–4. (PDF 1906 kb)

Supplementary Data Set 1

Original mass spectrometry data. (XLSX 56 kb)

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Teixeira, P., Kmiec, B., Branca, R. et al. A multi-step peptidolytic cascade for amino acid recovery in chloroplasts. Nat Chem Biol 13, 15–17 (2017). https://doi.org/10.1038/nchembio.2227

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