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The Taf14 YEATS domain is a reader of histone crotonylation

Nature Chemical Biology volume 12, pages 396–398 (2016)Cite this article

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Abstract

The discovery of new histone modifications is unfolding at startling rates; however, the identification of effectors capable of interpreting these modifications has lagged behind. Here we report the YEATS domain as an effective reader of histone lysine crotonylation, an epigenetic signature associated with active transcription. We show that the Taf14 YEATS domain engages crotonyllysine via a unique π–π–π-stacking mechanism and that other YEATS domains have crotonyllysine-binding activity.

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Figure 1: Structural mechanism for the recognition of H3K9cr.
Figure 2: H3K9cr is a selective target of the Taf14 YEATS domain.

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Acknowledgements

We thank L. Aponte-Collazo for help with experiments and M. Bedford, K. Chua, D. Stillman, and J. Tyler for kindly providing some original DNA constructs of YEATS and bromodomains, as well as the yeast strains and extracts. This work was supported by NIH grants R01 GM106416 and GM100907 to T.G.K. and GM110058 to B.D.S. and by CPRIT RP160237 to X.S. F.H.A. is supported by the NIH grant T32AA007464, E.K.S. is supported by the NIH grant K12-GM000678, S.A.S. is supported by a UNC Lineberger Cancer Center Postdoctoral Fellowship Award, and A.G. is supported by the CPRIT Research Training grant RP140106.

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Author notes

  1. Forest H Andrews and Stephen A Shinsky: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado, USA

    Forest H Andrews & Tatiana G Kutateladze

  2. Department of Biochemistry & Biophysics, the University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA

    Stephen A Shinsky, Erin K Shanle, Joseph B Bridgers, Ian K Tsun, Krzysztof Krajewski & Brian D Strahl

  3. Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center, Houston, Texas, USA

    Anneliese Gest & Xiaobing Shi

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  1. Forest H Andrews
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Contributions

F.H.A., S.A.S., E.K.S., J.B.B., A.G., I.K.T., and K.K. performed experiments and, together with X.S., B.D.S., and T.G.K., analyzed the data. F.H.A., S.A.S., B.D.S., and T.G.K. wrote the manuscript with input from all authors.

Corresponding authors

Correspondence to Brian D Strahl or Tatiana G Kutateladze.

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The authors declare no competing financial interests.

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Supplementary Results, Supplementary Figures 1–7 and Supplementary Tables 1 and 2. (PDF 13029 kb)

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Andrews, F., Shinsky, S., Shanle, E. et al. The Taf14 YEATS domain is a reader of histone crotonylation. Nat Chem Biol 12, 396–398 (2016). https://doi.org/10.1038/nchembio.2065

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