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N-terminal domains mediate [2Fe-2S] cluster transfer from glutaredoxin-3 to anamorsin

Nature Chemical Biology volume 11, pages 772778 (2015) | Download Citation

Abstract

In eukaryotes, cytosolic monothiol glutaredoxins are proteins implicated in intracellular iron trafficking and sensing via their bound [2Fe-2S] clusters. We define a new role of human cytosolic monothiol glutaredoxin-3 (GRX3) in transferring its [2Fe-2S] clusters to human anamorsin, a physical and functional protein partner of GRX3 in the cytosol, whose [2Fe-2S] cluster–bound form is involved in the biogenesis of cytosolic and nuclear Fe-S proteins. Specific protein recognition between the N-terminal domains of the two proteins is the mandatory requisite to promote the [2Fe-2S] cluster transfer from GRX3 to anamorsin.

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Acknowledgements

We thank A. Gallo (CERM) for assistance in recording the EPR spectra. This work was supported by Ente Cassa di Risparmio (Grant ID no. 2013/7101); the Ministero dell'Istruzione, dell'Università e della Ricerca (Grant ID number: CTN01_00177_962865); the European Integrated Structural Biology Infrastructure (INSTRUCT), which is part of the European Strategy Forum on Research Infrastructures; and national member subscriptions.

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Affiliations

  1. Magnetic Resonance Center CERM, University of Florence, Florence, Italy.

    • Lucia Banci
    • , Simone Ciofi-Baffoni
    • , Karolina Gajda
    • , Riccardo Muzzioli
    • , Riccardo Peruzzini
    •  & Julia Winkelmann
  2. Department of Chemistry, University of Florence, Florence, Italy.

    • Lucia Banci
    • , Simone Ciofi-Baffoni
    •  & Riccardo Muzzioli

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Contributions

The work plan was conceived and designed by L.B. and S.C.-B.; R.M. and K.G. produced protein constructs of anamorsin and GRX3; L.B. and S.C.-B. planned the experiments; J.W. and R.P. performed and analyzed NMR data; K.G., J.W. and R.M. performed and analyzed EPR and UV-vis experiments. The manuscript was drafted by L.B. and S.C.-B. and revised by all authors.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Lucia Banci or Simone Ciofi-Baffoni.

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DOI

https://doi.org/10.1038/nchembio.1892

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