Chromatin-level regulation of biosynthetic gene clusters

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  • A Corrigendum to this article was published on 01 September 2009

Abstract

Loss-of-function Aspergillus nidulans CclA, a Bre2 ortholog involved in histone H3 lysine 4 methylation, activated the expression of cryptic secondary metabolite clusters in A. nidulans. One new cluster generated monodictyphenone, emodin and emodin derivatives, whereas a second encoded two anti-osteoporosis polyketides, F9775A and F9775B. Modification of the chromatin landscape in fungal secondary metabolite clusters allows for a simple technological means to express silent fungal secondary metabolite gene clusters.

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Figure 1: Identification of monodictyphenone and its gene cluster.
Figure 2: Identification of F9775 and its gene cluster.

Change history

  • 18 August 2009

    In the version of this article initially published, the fourth author's last name is misspelled. The author's name should read "Yazmid Reyes-Dominguez." The error has been corrected in the HTML and PDF versions of the article.

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Acknowledgements

This research was funded in part by the US National Science Foundation grant MCB-0236393 to N.P.K., National Institutes of Health grant GM084077 to N.P.K., B.R.O. and C.C.C.W., and GM031837 to B.R.O. Work in Vienna was supported by grant P19731-B11 from the Austrian Science Fund (FWF) to J.S.

Author information

J.W.B. contributed to design and execution of experiments and to writing the manuscript. Y.-M.C., J.F.S. and H.-C.L. were involved in metabolite analysis of Aspergillus strains by LC-MS and in the isolation and structural determination of monodictyphenone, emodins and F9755A/B by one- and two-dimensional NMR. Y.R.-D. conducted the ChIP analysis. K.W. was involved in obtaining LC/MS data. E.S. and A.D.D. were involved in generating Aspergillus mutant strains. B.R.O., N.P.K., J.S. and C.C.C.W. contributed to design and writing.

Correspondence to Clay C C Wang or Nancy P Keller.

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