In vivo, hydrogenases require maturases for active site incorporation. However, in vitro, an active site model with limited catalytic activity could be incorporated into the apo form of [FeFe]-hydrogenase without the aid of maturases, generating enzyme with native activity.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Esselborn, J. et al. Nat. Chem. Biol. 10.1038/nchembio.1311 (11 Aug 2013).
Madden, C. et al. J. Am. Chem. Soc. 134, 1577–1582 (2012).
Peters, J.W., Lanzilotta, W.N., Lemon, B.J. & Seefeldt, L.C. Science 282, 1853–1858 (1998).
Posewitz, M.C. et al. J. Biol. Chem. 279, 25711–25720 (2004).
McGlynn, S.E. et al. FEBS Lett. 582, 2183–2187 (2008).
Tard, C. & Pickett, C.J. Chem. Rev. 109, 2245–2274 (2009).
Berggren, G. et al. Nature 499, 66–69 (2013).
Li, H. & Rauchfuss, T.B. J. Am. Chem. Soc. 124, 726–727 (2002).
Darensbourg, M.Y., Lyon, E.J., Zhao, X. & Georgakaki, I.P. Proc. Natl. Acad. Sci. USA 100, 3683–3688 (2003).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Roy, S., Jones, A. Cutting out the middleman. Nat Chem Biol 9, 603–605 (2013). https://doi.org/10.1038/nchembio.1322
Published:
Issue Date:
DOI: https://doi.org/10.1038/nchembio.1322
This article is cited by
-
Degree-preserving network growth
Nature Physics (2022)
-
The plasticity of redox cofactors: from metalloenzymes to redox-active DNA
Nature Reviews Chemistry (2018)